Nakajima Yasushi, Fiorellini Joseph P, Kim David M, Weber Hans P
Department of Periodontics, Penn School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Int J Periodontics Restorative Dent. 2007 Apr;27(2):151-9.
The purpose of this study was to evaluate the new bone formation and biodegradability of various bone fillers placed underneath a nonresorbable membrane (expanded polytetrafluoroethylene [e-PTFE]) in standardized defects in a beagle dog model. Mandibular premolars and first molars were extracted bilaterally in six adult female beagle dogs. Three months after the extractions, six rectangular defects (4 mm apicocoronally, 7 mm mesiodistally, and the entire width of the ridge buccolingually) were surgically created in the alveolar processes. All defects were covered with an e-PTFE membrane and stabilized buccally with stainless steel fixation screws. Five different bone fillers were placed under the membranes in a random configuration in each dog: autogenous bone, demineralized freeze-dried bone, anorganic bovine bone, tricalcium phosphate granules, and collagen sponge. One site in each animal was treated with the e-PTFE membrane alone. Following an 8-month healing period, a bone core was harvested from each grafted site using a trephine drill. Samples from each of the grafted sites were evaluated histologically and histometrically for their bone regenerative potential. The result of this study demonstrated a positive effect of bone fillers on bone regeneration when combined with a nonresorbable membrane; however, no significant differences were found between the various bone fillers in terms of their regenerative potential.
本研究的目的是在比格犬模型的标准化缺损中，评估置于不可吸收膜（膨体聚四氟乙烯[e-PTFE]）下方的各种骨填充材料的新骨形成和生物降解性。对6只成年雌性比格犬双侧拔除下颌前磨牙和第一磨牙。拔牙3个月后，在牙槽突上手术制造6个矩形缺损（根尖向冠方4mm，近远中向7mm，颊舌向为牙槽嵴的全宽）。所有缺损均用e-PTFE膜覆盖，并用不锈钢固定螺钉在颊侧固定。在每只犬中，将5种不同的骨填充材料以随机方式置于膜下：自体骨、脱矿冻干骨、无机牛骨、磷酸三钙颗粒和胶原海绵。每只动物的一个部位仅用e-PTFE膜处理。经过8个月的愈合期后，用环钻从每个移植部位获取骨芯。对每个移植部位的样本进行组织学和组织计量学评估，以确定其骨再生潜力。本研究结果表明，骨填充材料与不可吸收膜联合使用时对骨再生有积极作用；然而，各种骨填充材料在再生潜力方面未发现显著差异。
