Cassagne C, Juguelin H, Boiron F
Institut de Biochimie Cellulaire et Neurochimie du CNRS, Université de Bordeaux II, France.
Biochim Biophys Acta. 1991 Nov 18;1070(1):119-26. doi: 10.1016/0005-2736(91)90153-y.
The partition of 0.3 nmol of [1-14C]oleoyl-CoA in the microsomes (10 micrograms proteins) from mouse sciatic nerves is unaffected by the presence of lysophospholipids and is about 45% of the total oleoyl-CoA (77% of the acylglycerophosphocholine partition in the membrane). The concentration of both oleoyl-CoA and acylglycerophosphocholine is over 1 mM in the membrane. There is a selective acyl transfer from acyl-CoA to lysolipid acceptors (oleoyl greater than myristoyl, palmitoyl, stearoyl much greater than eicosanoyl greater than docosanoyl, tetracosanoyl). The exogenous acyl acceptors are acylglycerophosphocholine and acylglycerophosphoinositol and to a lesser extent acylglycerophosphoethanolamine, but not acylglycerophosphoserine. A PC formation from acylGPC in the absence of exogenous acyl donors or from oleoyl-CoA in the absence of exogenous acyl acceptor was also observed.
0.3纳摩尔的[1-¹⁴C]油酰辅酶A在小鼠坐骨神经微粒体（10微克蛋白质）中的分配不受溶血磷脂存在的影响，约占总油酰辅酶A的45%（占膜中酰基甘油磷酸胆碱分配的77%）。膜中油酰辅酶A和酰基甘油磷酸胆碱的浓度均超过1毫摩尔/升。存在从酰基辅酶A到溶血脂质受体的选择性酰基转移（油酰大于肉豆蔻酰、棕榈酰、硬脂酰远大于花生四烯酰大于二十二碳酰、二十四碳酰）。外源性酰基受体是酰基甘油磷酸胆碱和酰基甘油磷酸肌醇，酰基甘油磷酸乙醇胺的程度较小，但不是酰基甘油磷酸丝氨酸。在没有外源性酰基供体的情况下，也观察到由酰基甘油磷酸胆碱形成磷脂酰胆碱，或者在没有外源性酰基受体的情况下，由油酰辅酶A形成磷脂酰胆碱。
