Juguelin H, Bessoule J J, Cassagne C
Institut de Biochimie Cellulaire et de Neurochimie du CNRS, Université de Bordeaux II, France.
Biochim Biophys Acta. 1991 Sep 10;1068(1):41-51. doi: 10.1016/0005-2736(91)90058-g.
We have measured the partition of stearoyl-CoA and oleoyl-CoA between an aqueous phase and the microsomes from mouse sciatic nerves. A method of microultracentrifugation was used which allowed us to study separately the aqueous phase and the biological membranes. We observed that the partition is dependent upon the amount of acyl-CoAs and membrane proteins but seems to be independent of time. A theoretical analysis of these data allowed interpretation of the binding and release in terms of acyl-CoA surface density in the vesicles. We have also analyzed the fate of the membrane-bound acyl-CoAs. We show that, whereas the apparent partition does not seem to vary, the hydrolysis of the membrane-bound acyl-CoAs followed by the release of free fatty acids from the membrane leads to a modification of the partition of acyl-CoAs between the membrane and the aqueous phase. We propose that there is a constant partition of the aliphatic chains (acyl-CoAs + free fatty acids).
我们已经测量了硬脂酰辅酶A和油酰辅酶A在水相和小鼠坐骨神经微粒体之间的分配情况。采用了一种微量超速离心方法，使我们能够分别研究水相和生物膜。我们观察到，这种分配取决于酰基辅酶A和膜蛋白的量，但似乎与时间无关。对这些数据的理论分析使得能够根据囊泡中酰基辅酶A的表面密度来解释结合和释放情况。我们还分析了膜结合酰基辅酶A的去向。我们表明，虽然表观分配似乎没有变化，但膜结合酰基辅酶A的水解以及随后游离脂肪酸从膜中的释放导致酰基辅酶A在膜和水相之间的分配发生改变。我们提出脂肪链（酰基辅酶A + 游离脂肪酸）存在恒定的分配。
