Canola Kriss, Arsenijevic Yvan
Unit of Gene Therapy and Stem Cell Biology, Jules Gonin Eye Hospital, Lausanne University, Lausanne, Switzerland.
Neuroreport. 2007 Jun 11;18(9):851-5. doi: 10.1097/WNR.0b013e32815277c1.
Cell transplantation is an active field of research to replace lost cells in retinal dystrophies to potentially restore visual function. We hypothesized that in-vitro differentiated retinal stem cells would integrate the appropriate retinal layer and differentiate into photoreceptors when transplanted during development. Here we show that retinal stem cells driven to the photoreceptor fate start to incorporate the retina and express photoreceptor markers but do not survive. Nevertheless surviving grafted cells express the glial marker glial fibrillary acidic protein and incorporate the ganglion cell layer as well as the inner plexiform layer. These results suggest that the maturation state of the photoreceptors is primordial to obtain robust incorporation and that a fine tuning of retinal stem cells differentiation should provide adequate cells for transplantation.
细胞移植是一个活跃的研究领域，旨在替代视网膜营养不良中丢失的细胞，以潜在地恢复视觉功能。我们假设，体外分化的视网膜干细胞在发育过程中移植时，将整合到适当的视网膜层并分化为光感受器。在这里，我们表明，被诱导为光感受器命运的视网膜干细胞开始融入视网膜并表达光感受器标记物，但无法存活。然而，存活的移植细胞表达神经胶质标记物胶质纤维酸性蛋白，并融入神经节细胞层以及内网状层。这些结果表明，光感受器的成熟状态对于实现稳固的整合至关重要，并且对视网膜干细胞分化的精细调节应该能够为移植提供足够的细胞。
