Miyake Daisuke, Ichiki Shin-ichi, Tanabe Miyako, Oda Takahiro, Kuroda Hisao, Nishihara Hirofumi, Sambongi Yoshihiro
Graduate School of Biosphere Science, Hiroshima University, CREST of Japan Science and Technology Corporation, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8528, Japan,
Arch Microbiol. 2007 Aug;188(2):199-204. doi: 10.1007/s00203-007-0244-7. Epub 2007 May 22.
The moderately thermophilic Betaproteobacterium, Hydrogenophilus thermoluteolus, not only oxidizes hydrogen, the principal electron donor for growth, but also sulfur compounds including thiosulfate, a process enabled by sox genes. A periplasmic extract of H. thermoluteolus showed significant thiosulfate oxidation activity. Ten genes apparently involved in thiosulfate oxidation (soxEFCDYZAXBH) were found on a 9.7-kb DNA fragment of the H. thermoluteolus chromosome. The proteins SoxAX, which represent c-type cytochromes, were co-purified from the cells of H. thermoluteolus; they enhanced the thiosulfate oxidation activity of the periplasmic extract when added to the latter.
嗜温性中等的β-变形菌嗜热栖热氢菌，不仅能氧化氢气（生长的主要电子供体），还能氧化包括硫代硫酸盐在内的含硫化合物，这一过程由sox基因促成。嗜热栖热氢菌的周质提取物表现出显著的硫代硫酸盐氧化活性。在嗜热栖热氢菌染色体的一个9.7 kb DNA片段上发现了10个明显参与硫代硫酸盐氧化的基因（soxEFCDYZAXBH）。代表c型细胞色素的蛋白质SoxAX是从嗜热栖热氢菌细胞中共同纯化出来的；将它们添加到周质提取物中时，能增强后者的硫代硫酸盐氧化活性。
