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傣药传统药用植物尾叶巴豆的分子鉴定

Molecular authentication of the traditional dai medicinal plant Croton caudatus.

作者信息

Xue Heng-Gang, Zhou Song-Dong, He Xing-Jin, Yu Yan

机构信息

College of Life Science, Sichuan University, Chengdu, Sichuan, People's Republic of China.

出版信息

Planta Med. 2007 Jun;73(6):611-3. doi: 10.1055/s-2007-967197. Epub 2007 May 22.

DOI:10.1055/s-2007-967197
PMID:17516330
Abstract

Croton caudatus is an important species in Dai folk medicine. Some other Croton species, in particular, C. kongensis, C. cascarilloides, C. crassifolius, C. lachnocarpus and C. olivaceus are often marketed as C. caudatus, and thus, the therapeutic effects of C. caudatus are not achieved. The respective morphological features of the plants are similar, and they are not easy to distinguish morphologically from each other. In an attempt to find a method for discriminating among these species, we compared their nuclear ribosomal DNA internal transcribed spacer (ITS) region. Species-specific probes were derived from the ITS region of these species for species identification. This method provides effective and accurate identification of C. caudatus.

摘要

尾叶巴豆是傣药中的一种重要植物。其他一些巴豆属植物,特别是刚果巴豆、香叶巴豆、厚叶巴豆、毛果巴豆和油叶巴豆,常被当作尾叶巴豆出售,因此无法达到尾叶巴豆的治疗效果。这些植物各自的形态特征相似,在形态上不易相互区分。为了找到一种区分这些物种的方法,我们比较了它们的核糖体DNA内转录间隔区(ITS)。从这些物种的ITS区域获得了物种特异性探针用于物种鉴定。该方法为尾叶巴豆提供了有效且准确的鉴定。

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