Cecchini Cinzia, Cresci Alberto, Coman Maria Magdalena, Ricciutelli Massimo, Sagratini Gianni, Vittori Sauro, Lucarini Domenico, Maggi Filippo
University of Camerino, Faculty of Sciences and Technology, Department of Comparative Morphology and Biochemistry, Camerino, Italy.
Planta Med. 2007 Jun;73(6):564-6. doi: 10.1055/s-2007-967198. Epub 2007 May 22.
Methanol-acetone extracts from aerial parts of seven entities of the genus Hypericum [H. perforatum L. subsp. perforatum, H. perforatum L. subsp. veronense (Schrank) Ces., H. montanum L., H. hyssopifolium Chaix, H. hirsutum L., H. hircinum L. subsp. majus (Aiton) N. Robson, H. tetrapterum Fr.] growing in the Appennino Umbro-Marchigiano (central Italy) were tested in vitro for their antimicrobial activity against two Gram-positive bacteria (Staphylococcus aureus and Enterococcus faecalis), two Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and the yeast Candida albicans, by the Kirby-Bauer agar diffusion method. The two H. perforatum subspecies were particularly active against Gram-positive and Gram-negative bacteria, supporting the use of these plants in the traditional medicine of central Italy to treat wounds, skin and infectious diseases. However significant values of inhibition were given also by H. hirsutum against S. aureus and P. aeruginosa, and by H. hyssopifolium, H. hircinum subsp. majus and H. tetrapterum against C. albicans. These results suggest that hypericin and hyperforin are the main components responsible for antimicrobial activity, but not the only ones, as other extracts, showing a low level of these compounds, possess evident activity. Isolation of pure compounds from the most active extracts is in progress.
对生长在意大利中部翁布里亚 - 马尔凯亚诺亚平宁山脉的金丝桃属七个物种[贯叶连翘L. 亚种贯叶连翘、贯叶连翘L. 亚种维罗内塞（施兰克）切斯、山地金丝桃L.、细叶金丝桃柴克斯、毛金丝桃L.、臭金丝桃L. 亚种大花（艾顿）N. 罗布森、四翅金丝桃弗尔]地上部分的甲醇 - 丙酮提取物，采用 Kirby - Bauer 琼脂扩散法，在体外测试其对两种革兰氏阳性菌（金黄色葡萄球菌和粪肠球菌）、两种革兰氏阴性菌（大肠杆菌和铜绿假单胞菌）以及白色念珠菌的抗菌活性。贯叶连翘的两个亚种对革兰氏阳性菌和革兰氏阴性菌特别有活性，这支持了这些植物在意大利中部传统医学中用于治疗伤口、皮肤和传染病的用途。然而，毛金丝桃对金黄色葡萄球菌和铜绿假单胞菌，以及细叶金丝桃、臭金丝桃亚种大花和四翅金丝桃对白色念珠菌也给出了显著的抑制值。这些结果表明，金丝桃素和贯叶连翘素是抗菌活性的主要成分，但不是唯一成分，因为其他提取物虽然这些化合物含量低，但也具有明显活性。目前正在从活性最高的提取物中分离纯化合物。
