Okano Hideyuki, Tomita Naohide, Ikada Yoshito
International Innovation Center, Kyoto University, Kyoto, Japan.
Bioelectromagnetics. 2007 Sep;28(6):497-9. doi: 10.1002/bem.20330.
We designed this study to examine the effects of static magnetic fields (SMF; 120 mT [B(max)] and a maximum spatial magnetic flux gradient of 21 mT/mm) on inhibited tubular formation when treated with human transforming growth factor (TGF)-beta1 at a relatively high concentration (5 ng/ml). Three experimental groups of 25 samples each were examined: (1) sham exposure alone (control); (2) sham-exposure with TGF-beta1; (3) SMF exposure with TGF-beta1. The SMF or sham exposure was carried out for 10 days. Photomicrographs of tubular cells, immunostained with an anti-platelet-endothelial cell adhesion molecule-1 (PECAM-1 [CD31]) antibody as a pan-endothelial marker, were analyzed after the 10-day culture. SMF was found to significantly reverse the inhibition of TGF-beta1 on tubular formation in terms of the area density and length of tubules (arteriogenesis) in the peripheral part of the wells, compared with the TGF-beta1 treatment alone. These findings suggest that one of the possible exogenous factors for arteriogenesis might involve 'magnetic force' (the product of the magnetic flux density, the magnetic gradient, and the volume susceptibility of the cells) because values are much larger in the peripheral part than in the central part.
我们设计了这项研究，以检验静磁场（SMF；120 mT [B(max)]，最大空间磁通梯度为21 mT/mm）在相对高浓度（5 ng/ml）的人转化生长因子（TGF）-β1处理时对管状形成抑制的影响。对三个实验组进行了检测，每组25个样本：（1）仅假暴露（对照）；（2）假暴露加TGF-β1；（3）SMF暴露加TGF-β1。SMF或假暴露持续10天。在10天培养后，分析用抗血小板内皮细胞黏附分子-1（PECAM-1 [CD31]）抗体作为全内皮标志物进行免疫染色的管状细胞的显微照片。与单独TGF-β1处理相比，发现SMF在孔周边部分的小管面积密度和长度（动脉生成）方面显著逆转了TGF-β1对管状形成的抑制。这些发现表明，动脉生成的一个可能的外源性因素可能涉及“磁力”（磁通密度、磁梯度和细胞体积磁化率的乘积），因为周边部分的值比中心部分大得多。
