与酵母自主复制序列复制增强子具有同源性的乙肝病毒DNA区域。

Region of hepatitis B virus DNA with a homology to the yeast ARS replication enhancer.

作者信息

Legchilina S P, Efimenko I G, Shlyakhtenko L S, Arman I P

机构信息

Institute of Molecular Genetics, Academy of Sciences of the USSR, Moscow.

出版信息

Biomed Sci. 1991;2(3):298-301.

PMID:1751764

Abstract

A computer analysis of the primary sequence of hepatitis B virus (HBV) subtype ayw DNA, cloned within the pVG2 recombinant plasmid, which raises its stability in Saccharomyces cerevisiae transformants, was performed. This revealed that the structure of the HBV DNA has: two bends in the termination regions of the HBs and HBc genes, and multiple sequences with a high degree of homology to the ARS (autonomously replicating sequence) core consensus in this region of the HBs gene. DNA fragments from the HBs region (330 bp) and from the HBc region (378 bp) have an abnormal electrophoretic mobility in 8% polyacrylamide gels. The similarity of the structural motifs in the stop-region of HBs gene with the B-domain of the S. cerevisiae ARS element is discussed.

摘要

对克隆于pVG2重组质粒中的乙型肝炎病毒（HBV）ayw亚型DNA的一级序列进行了计算机分析，该重组质粒提高了其在酿酒酵母转化子中的稳定性。结果显示，HBV DNA的结构具有：在HBs和HBc基因的终止区域有两个弯曲，以及在HBs基因的该区域与ARS（自主复制序列）核心共有序列具有高度同源性的多个序列。来自HBs区域（330 bp）和HBc区域（378 bp）的DNA片段在8%聚丙烯酰胺凝胶中具有异常的电泳迁移率。文中讨论了HBs基因终止区域的结构基序与酿酒酵母ARS元件B结构域的相似性。