Rogaev E I, Shlenskii A B
National Mental Research Centre, Academy of Medical Sciences of the USSR, Moscow.
Biomed Sci. 1991;2(3):311-3.
It was postulated that similar genetic elements that are 'hot spots' for genetic variation might exist in both the HIV-1 and the human genome. To test this possibility a short repeated sequence from a region of variability in the HIV-1 glycoprotein (env) gene was amplified and used as a probe for blot hybridization with human genome DNA. Human genomic regions were hybridized and characterized by a set of polymorphic restriction DNA fragments. The pattern of the restriction fragments was individual specific. Thus a DNA probe from the HIV-1 env gene can serve as a genetic marker for hybridization with human genome regions and for the identification of individuals.
据推测，在HIV-1和人类基因组中可能存在作为遗传变异“热点”的相似遗传元件。为了检验这种可能性，从HIV-1糖蛋白（env）基因的可变区扩增出一段短重复序列，并用作与人类基因组DNA进行印迹杂交的探针。人类基因组区域通过一组多态性限制性DNA片段进行杂交和表征。限制性片段的模式具有个体特异性。因此，来自HIV-1 env基因的DNA探针可作为与人类基因组区域杂交以及个体识别的遗传标记。
