Nishida K, Kimura Y, Kawasaki T, Fujie M, Yamada T
Graduate School of ADSM, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima, 739-8527, Japan.
Virology. 1999 Mar 15;255(2):376-84. doi: 10.1006/viro.1998.9595.
A physical map of the Chlorella virus CVK2 genomic DNA has been constructed based on a cosmid contig covering the entire genomic region. By using Southern blot analysis with 22 gene probes, the gene arrangement along the genome was compared between CVK2 and PBCV-1, the prototypic member of Phycodnaviridae, whose genomic sequence is now available. The major rearrangements were (1) an insertion of a 20-kbp region around the left end of CVK2 DNA, (2) a duplication of the gene for major capsid protein in CVK2 DNA, (3) deletions/insertions of some open reading frames, and (4) divergence in the terminal inverted repeat sequences. Despite these changes, extensive colinearity was revealed between most of the genes along the CVK2 and PBCV-1 genomes. These data imply that the Chlorella virus genome has an overall high degree of genomic stability, encompassing specific islands of rearrangements.
基于覆盖整个基因组区域的黏粒重叠群构建了小球藻病毒CVK2基因组DNA的物理图谱。通过使用22个基因探针进行Southern杂交分析，比较了CVK2与Phycodnaviridae科的原型成员PBCV-1沿基因组的基因排列情况，PBCV-1的基因组序列现已可得。主要的重排包括：（1）CVK2 DNA左端约20kbp区域的插入；（2）CVK2 DNA中主要衣壳蛋白基因的重复；（3）一些开放阅读框的缺失/插入；（4）末端反向重复序列的差异。尽管有这些变化，但CVK2和PBCV-1基因组中大多数基因之间仍显示出广泛的共线性。这些数据表明小球藻病毒基因组总体上具有高度的基因组稳定性，其中包含特定的重排区域。
