铜绿假单胞菌对环丙沙星耐药突变株中ampC表达增加。
Increased expression of ampC in Pseudomonas aeruginosa mutants selected with ciprofloxacin.
作者信息
Wolter Daniel J, Schmidtke Amber J, Hanson Nancy D, Lister Philip D
机构信息
Center for Research in Anti-Infectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, NE 68178, USA.
出版信息
Antimicrob Agents Chemother. 2007 Aug;51(8):2997-3000. doi: 10.1128/AAC.00111-07. Epub 2007 May 21.
Abstract
Two Pseudomonas aeruginosa mutants exhibiting increased expression of ampC were selected during exposure to ciprofloxacin. These mutants also exhibited significant increases in mexCD-oprJ expression, but further studies failed to show a link between the increased expression of mexCD-oprJ and ampC. Increased ampC expression was not related to mutations within ampR, the ampC-ampR intergenic region, ampD, ampDh2, or ampDh3 or to changes in the levels of expression of these amidase genes. However, ampD complementation restored wild-type levels of ampC expression and ceftazidime susceptibility, suggesting alternative mechanisms of ampC regulation.
摘要
在接触环丙沙星期间，筛选出了两株表现出ampC表达增加的铜绿假单胞菌突变体。这些突变体的mexCD-oprJ表达也显著增加，但进一步研究未能表明mexCD-oprJ表达增加与ampC之间存在联系。ampC表达增加与ampR、ampC-ampR基因间区域、ampD、ampDh2或ampDh3内的突变无关，也与这些酰胺酶基因的表达水平变化无关。然而，ampD互补恢复了ampC表达的野生型水平和头孢他啶敏感性，提示ampC调控的其他机制。
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