Horie H, Fukuda N, Bando Y
Department of Physiology, School of Medicine, Yokohama City University, Japan.
Neuroreport. 1991 Sep;2(9):521-4. doi: 10.1097/00001756-199109000-00006.
The culture of hepatocytes dissociated from adult mice by the collagenase perfusion method enables neurons or neuronal tissues to be cultured with hepatocytes or in hepatocyte-conditioned media. Co-culture with hepatocytes or hepatocyte-conditioned media enhances neurite regeneration and their survival from nerve-transected terminals of dorsal root ganglia with nerve fibers dissected from adult and aged mice. Hepatocytes secrete a factor which enhances not only neurite regeneration but also neurite survival. Activities of other known neurotrophic factors were not as crucial as those of the hepatocyte-conditioned medium, suggesting that this factor may differ from other trophic ones.
通过胶原酶灌注法从成年小鼠分离的肝细胞培养物能够使神经元或神经组织与肝细胞一起培养，或在肝细胞条件培养基中培养。与肝细胞或肝细胞条件培养基共培养可增强背根神经节神经横断末端的神经突再生及其存活，这些神经横断末端的神经纤维取自成年和老年小鼠。肝细胞分泌一种不仅能增强神经突再生而且能增强神经突存活的因子。其他已知神经营养因子的活性不如肝细胞条件培养基的活性关键，这表明该因子可能与其他营养因子不同。
