Horie H, Bando Y, Chi H, Takenaka T
Department of Physiology, School of Medicine, Yokohama City University, Japan.
Neurosci Lett. 1991 Jan 2;121(1-2):125-8. doi: 10.1016/0304-3940(91)90665-g.
Dorsal root ganglia with nerve fibers, dissected from 3-month-old young adult mice and 26-month-old aged mice, were embedded in collagen gel. In the young adult mice regenerating neurites appeared from nerve-transected terminals after 12-24 h in culture, regardless of the length of fibers, but in the aged mice, regenerating neurites were first seen after 12-48 h. Administration of nerve growth factor to the explants markedly enhanced the growth of regenerating neurites from both the young adult and aged mice.
从3个月大的成年小鼠和26个月大的老年小鼠中分离出带有神经纤维的背根神经节，将其嵌入胶原凝胶中。在成年小鼠中，无论纤维长度如何，培养12 - 24小时后，神经横断末端就会出现再生神经突，但在老年小鼠中，再生神经突最早在12 - 48小时后出现。向外植体施用神经生长因子显著增强了成年小鼠和老年小鼠再生神经突的生长。
