Nagae Masateru, Ikeda Takumi, Mikami Yasuo, Hase Hitoshi, Ozawa Hitoshi, Matsuda Ken-Ichi, Sakamoto Hirotaka, Tabata Yasuhiko, Kawata Mitsuhiro, Kubo Toshikazu
Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Tissue Eng. 2007 Jan;13(1):147-58. doi: 10.1089/ten.2006.0042.
This study evaluated the regenerative effects of platelet-rich plasma (PRP) for the degenerated intervertebral disc (IVD) in vivo. After induction of IVD degeneration in rabbits, we prepared PRP by centrifuging blood obtained from these rabbits. These PRP were injected into the nucleus pulposus (NP) of the degenerated IVDs after impregnation into gelatin hydrogel microspheres that can immobilize PRP growth factors physiochemically and release them in a sustained manner with the degradation of the microspheres. As controls, microspheres impregnated with phosphate-buffered saline (PBS) and PRP without microspheres were similarly injected. Histologically, notable progress in IVD degeneration with time courses was observed in the PBS control, PRP-only, and sham groups. In contrast, progress was remarkably suppressed over the 8-week period in the PRP group. Moreover, in immunohistochemistry, intense immunostaining for proteoglycan in the NP and inner layer of the annulus fibrosus was observed 8 weeks after administration of PRP-impregnated microspheres. Almost all microspheres were indistinct 8 weeks after the injection, and there were no apparent side effects in this study. Our results suggest that the combined administration of PRP and gelatin hydrogel microspheres into the IVD may be a promising therapeutic modality for IVD degeneration.
本研究在体内评估了富血小板血浆（PRP）对退变椎间盘（IVD）的再生作用。在诱导兔椎间盘退变后，我们通过离心从这些兔采集的血液制备了PRP。将这些PRP在浸渍到可通过物理化学方式固定PRP生长因子并随着微球降解而持续释放它们的明胶水凝胶微球中后，注入退变IVD的髓核（NP）。作为对照，同样注射浸渍有磷酸盐缓冲盐水（PBS）的微球和未加微球的PRP。组织学上，在PBS对照组、单纯PRP组和假手术组中观察到IVD退变随时间进程有明显进展。相比之下，PRP组在8周期间进展明显受到抑制。此外，免疫组织化学显示，在注射PRP浸渍微球8周后，在NP和纤维环内层观察到蛋白聚糖的强烈免疫染色。注射8周后几乎所有微球都不清楚了，并且在本研究中没有明显的副作用。我们的结果表明，将PRP和明胶水凝胶微球联合注入IVD可能是一种有前景的治疗IVD退变的方式。
