Matsumoto Daisuke, Sato Katsujiro, Gonda Koichi, Takaki Yasuyuki, Shigeura Tomokuni, Sato Takahiro, Aiba-Kojima Emiko, Iizuka Fumiko, Inoue Keita, Suga Hirotaka, Yoshimura Kotaro
Department of Plastic Surgery, University of Tokyo School of Medicine, Tokyo, Japan.
Tissue Eng. 2006 Dec;12(12):3375-82. doi: 10.1089/ten.2006.12.3375.
Injective transfer of autologous aspirated fat is a popular option for soft tissue augmentation, but several issues require attention, including unpredictability and a low survival rate due to partial necrosis. In this study, histologic features and yield of adipose-derived stromal (stem) cells (ASCs) were compared between human aspirated fat and excised whole fat. Aspirated fat contained fewer large vascular structures, and ASC yield was lower in aspirated fat. Aspirated fat was transplanted subcutaneously into severe combined immunodeficiency mice with (cell-assisted lipotransfer; CAL) or without (non-CAL) vascular stromal fractions containing ASCs isolated from adipose tissue. The CAL fat survived better (35% larger on average) than non-CAL fat, and microvasculature was detected more prominently in CAL fat, especially in the outer layers. DiI-labeled vascular stromal fraction cells were found between adipocytes and in the connective tissue in CAL fat, and some of these cells were immunopositive for von Willebrand factor, suggesting differentiation into vascular endothelial cells. Another experiment that used vascular stromal fractions taken from green fluorescent protein rats also suggested that ASCs differentiated into vascular endothelial cells and contributed to neoangiogenesis in the acute phase of transplantation. These findings may partly explain why transplanted aspirated fat does not survive well and suggest clinical potential of the CAL method for soft tissue augmentation.
自体抽吸脂肪的注射移植是软组织填充的一种常用方法，但有几个问题需要注意，包括不可预测性以及因部分坏死导致的低存活率。在本研究中，对人抽吸脂肪和切除的整块脂肪之间的脂肪来源基质（干）细胞（ASC）的组织学特征和产量进行了比较。抽吸脂肪中大型血管结构较少，抽吸脂肪中的ASC产量较低。将抽吸脂肪皮下移植到严重联合免疫缺陷小鼠体内，分别采用含有从脂肪组织中分离出的ASC的血管基质成分（细胞辅助脂肪移植；CAL）或不采用（非CAL）。CAL脂肪比非CAL脂肪存活得更好（平均大35%），并且在CAL脂肪中，尤其是在外层，微血管更明显。在CAL脂肪的脂肪细胞之间和结缔组织中发现了DiI标记的血管基质成分细胞，其中一些细胞对血管性血友病因子呈免疫阳性，表明其分化为血管内皮细胞。另一项使用从绿色荧光蛋白大鼠获取的血管基质成分的实验也表明，ASC在移植急性期分化为血管内皮细胞并促进新生血管形成。这些发现可能部分解释了为什么移植的抽吸脂肪存活不佳，并提示了CAL方法在软组织填充方面的临床潜力。
