Rivoire N, Ravololonandriana P, Rasolonavalona T, Martin A, Portaels F, Ramarokoto H, Rasolofo Razanamparany V
Unité des Mycobactéries, Institut Pasteur de Madagascar, Antananarivo, Madagascar.
Int J Tuberc Lung Dis. 2007 Jun;11(6):683-8.
Multidrug-resistant (MDR) tuberculosis (TB) can jeopardise the success of national TB control programmes. Rapid, simple drug susceptibility tests applicable in developing countries would allow earlier treatment of patients with MDR infections.
To test the feasibility and performance of the resazurin microtitre assay (REMA) as an indirect test for detecting isoniazid (INH) and rifampicin (RMP) resistance of Mycobacterium tuberculosis strains in Madagascar.
Study comparing the sensitivity and specificity of the REMA plate test with the Löwenstein-Jensen proportion method for determining the resistance of M. tuberculosis strains to INH and RMP.
The sensitivity and specificity of the resazurin test were studied in 77 strains and were respectively 95% and 97.3% for the detection of INH resistance, and 95% and 100% for the detection of RMP resistance. The sensitivity and specificity for the identification of MDR strains were respectively 89% and 100%.
The resazurin test is sensitive and specific enough for the detection of INH- and RMP-resistant strains. It is also easy to use, rapid and inexpensive, making it suitable for developing countries. Its usefulness for national drug resistance surveys should be assessed.
耐多药结核病（MDR-TB）会危及国家结核病控制规划的成效。适用于发展中国家的快速、简易药敏试验可使耐多药感染患者得到更早治疗。
检测刃天青微量滴定法（REMA）作为检测马达加斯加结核分枝杆菌菌株对异烟肼（INH）和利福平（RMP）耐药性的间接试验的可行性和性能。
比较REMA平板试验与罗氏培养基比例法检测结核分枝杆菌菌株对INH和RMP耐药性的敏感性和特异性的研究。
在77株菌株中研究了刃天青试验的敏感性和特异性，检测INH耐药性时分别为95%和97.3%，检测RMP耐药性时分别为95%和100%。鉴定耐多药菌株的敏感性和特异性分别为89%和100%。
刃天青试验对于检测对INH和RMP耐药的菌株具有足够的敏感性和特异性。它还易于使用、快速且廉价，适合发展中国家。应评估其在全国耐药性调查中的实用性。
