Gui Xiao-Hong, Xu Peng, Zhao Ming, Wang Li-Li, Wu Jie, Li Jing, Gao Qian, Mei Jian
Department of Tuberculosis, Shanghai Municipal Center for Disease Control & Prevention, Shanghai 200336, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2010 Jan;33(1):43-5.
to evaluate the ability of GenoType MTBDRplus Assay for detection of rifampin (RFP) and isoniazid (INH) resistance in Mycobacterium tuberculosis.
seventy-five clinical isolates were tested for traditional drug susceptibility testing and GenoType MTBDRplus assay. The results were compared to assess the sensitivity and specificity of GenoType MTBDRplus assay.
the sensitivity of GenoType MTBDRplus assays for detection of RFP and INH resistance were 98.0% and 86.5%, respectively. In pan-susceptible isolates of Mycobacterium tuberculosis, the specificity was 100% for both RFP resistance and INH resistance.
with its high sensitivity and specificity, GenoType MTBDRplus assay is a promising rapid method to detect INH and RFP resistant Mycobacterium tuberculosis strains in Shanghai.
评估GenoType MTBDRplus检测法检测结核分枝杆菌中利福平（RFP）和异烟肼（INH）耐药性的能力。
对75株临床分离株进行传统药敏试验和GenoType MTBDRplus检测法检测。比较结果以评估GenoType MTBDRplus检测法的敏感性和特异性。
GenoType MTBDRplus检测法检测RFP和INH耐药性的敏感性分别为98.0%和86.5%。在结核分枝杆菌全敏感分离株中，RFP耐药性和INH耐药性的特异性均为100%。
GenoType MTBDRplus检测法具有高敏感性和特异性，是上海检测耐INH和RFP结核分枝杆菌菌株的一种有前景的快速方法。
