Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E1.
Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta, Canada T6G 2V4.
Nat Commun. 2016 Oct 4;7:12947. doi: 10.1038/ncomms12947.
In the fight against drug-resistant bacteria, accurate and high-throughput detection is essential. Here, a bimaterial microcantilever with an embedded microfluidic channel with internal surfaces chemically or physically functionalized with receptors selectively captures the bacteria passing through the channel. Bacterial adsorption inside the cantilever results in changes in the resonance frequency (mass) and cantilever deflection (adsorption stress). The excitation of trapped bacteria using infrared radiation (IR) causes the cantilever to deflect in proportion to the infrared absorption of the bacteria, providing a nanomechanical infrared spectrum for selective identification. We demonstrate the in situ detection and discrimination of Listeria monocytogenes at a concentration of single cell per μl. Trapped Escherichia coli in the microchannel shows a distinct nanomechanical response when exposed to antibiotics. This approach, which combines enrichment with three different modes of detection, can serve as a platform for the development of a portable, high-throughput device for use in the real-time detection of bacteria and their response to antibiotics.
在与耐药菌的斗争中，准确和高通量的检测至关重要。在这里，一种双材料微悬臂梁带有一个嵌入式微流道，其内部表面通过化学或物理方法功能化，带有选择性捕获通过通道的细菌的受体。细菌在悬臂梁内的吸附导致共振频率（质量）和悬臂梁挠度（吸附应力）的变化。使用红外辐射（IR）激发被困细菌会导致悬臂梁根据细菌的红外吸收而偏转，从而提供用于选择性识别的纳米机械红外光谱。我们在单细胞每 μl 的浓度下演示了李斯特菌的原位检测和区分。当暴露于抗生素时，微通道中的被困大肠杆菌表现出明显的纳米机械响应。这种结合了三种不同检测模式的富集方法，可以作为开发便携式、高通量设备的平台，用于实时检测细菌及其对抗生素的反应。
