单分子生物物理学：处于生物学、物理学与化学的交叉领域
Single-molecule biophysics: at the interface of biology, physics and chemistry.
作者信息
Deniz Ashok A, Mukhopadhyay Samrat, Lemke Edward A
机构信息
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
出版信息
J R Soc Interface. 2008 Jan 6;5(18):15-45. doi: 10.1098/rsif.2007.1021.
Abstract
Single-molecule methods have matured into powerful and popular tools to probe the complex behaviour of biological molecules, due to their unique abilities to probe molecular structure, dynamics and function, unhindered by the averaging inherent in ensemble experiments. This review presents an overview of the burgeoning field of single-molecule biophysics, discussing key highlights and selected examples from its genesis to our projections for its future. Following brief introductions to a few popular single-molecule fluorescence and manipulation methods, we discuss novel insights gained from single-molecule studies in key biological areas ranging from biological folding to experiments performed in vivo.
摘要
单分子方法已经发展成为探测生物分子复杂行为的强大且常用的工具，这得益于其独特的能力，即能够在不受系综实验固有平均化影响的情况下，探测分子结构、动力学和功能。本综述概述了新兴的单分子生物物理学领域，讨论了从其起源到我们对其未来预测的关键亮点和精选实例。在简要介绍了几种流行的单分子荧光和操纵方法之后，我们讨论了在从生物折叠到体内实验等关键生物学领域的单分子研究中获得的新见解。
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