Zhang Lin-Li, Lu Jing, Yu Shu-Qin, He Jian-Lin, Zhou Min, Xu Guang-Lin
Center of New Drug Research and Development, Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210097, China.
Yao Xue Xue Bao. 2007 Mar;42(3):252-6.
Acute lung injury (ALI) makes up a spectrum of disease that is commonly defined as "acute non-cardiogenic edematous lung injury". It may contribute to morbidity and mortality in the critically ill patient in the intensive care unit. ALI was induced by oleic acid in rabbits. During the experiment, blood samples were taken from cervical artery and subjected to blood-gas analysis at different time points after oleic acid injection. Shortly after the rabbits were killed at 3 hour after iv OA injection, bronchoalveolar lavage fluid (BALF) was colleted, and the concentrations of protein, platelet-activating factor (PAF), intercellular adhesion molecule-1 (ICAM-1), interleukin 8 (IL-8) in BALF were then measured by ELISA. The ratio of wet to dry weight (W/D) of left lung was calculated to assess alveolar edema. Lung tissue was fixed in formaldehyde and stained with HE, and examined under a light microscope. The OA-induced elevation of arterial blood oxygen pressure was inhibited, as well as PAF, ICAM-1, IL-8 in BALF in rupatadine group. Furthermore, rupatadine also decreased the concentration of protein in BALF and inhibited the increase of the W/D weight ratio significantly. Light microscopic findings showed that the damage in rupatadine groups was far less severe than that in OA model group. Pretreatment with rupatadine has a beneficial effect on acute lung injury induced by oleic acid in rabbits. The ultimate reduction of inflammatory factors was involved, at least in part, in the mechanism of action of rupatadine effects.
急性肺损伤（ALI）构成了一系列疾病，通常被定义为“急性非心源性肺水肿性肺损伤”。它可能导致重症监护病房中危重病患者的发病和死亡。在兔身上用油酸诱导ALI。实验过程中，于油酸注射后的不同时间点从颈总动脉采集血样并进行血气分析。在静脉注射油酸后3小时处死兔子后不久，收集支气管肺泡灌洗液（BALF），然后通过酶联免疫吸附测定法（ELISA）检测BALF中蛋白质、血小板活化因子（PAF）、细胞间黏附分子-1（ICAM-1）、白细胞介素8（IL-8）的浓度。计算左肺湿重与干重之比（W/D）以评估肺泡水肿。将肺组织用甲醛固定，进行苏木精-伊红（HE）染色，并在光学显微镜下检查。在卢帕他定组中，油酸诱导的动脉血氧分压升高以及BALF中的PAF、ICAM-1、IL-8均受到抑制。此外，卢帕他定还降低了BALF中蛋白质的浓度，并显著抑制了W/D重量比的增加。光学显微镜检查结果显示，卢帕他定组的损伤远比油酸模型组轻。卢帕他定预处理对兔油酸诱导的急性肺损伤具有有益作用。炎症因子的最终减少至少部分参与了卢帕他定作用的机制。
