Leosco Dario, Rengo Giuseppe, Iaccarino Guido, Sanzari Emma, Golino Luca, De Lisa Gabriella, Zincarelli Carmela, Fortunato Francesca, Ciccarelli Michele, Cimini Vincenzo, Altobelli Giovanna Giuseppina, Piscione Federico, Galasso Gennaro, Trimarco Bruno, Koch Walter J, Rengo Franco
Dipartimento di Medicina Clinica, Scienze Cardiovascolari ed Immunologiche, Federico II University, Via Pansini 5, Edificio 2, 80131 Naples, Italy.
J Gerontol A Biol Sci Med Sci. 2007 May;62(5):471-80. doi: 10.1093/gerona/62.5.471.
Downregulation of hypoxia-inducible factor 1 (HIF-1) and vascular endothelial growth factor (VEGF) are shown to be involved in age-dependent impairment of angiogenesis. In this study, we explore whether prior exercise is able to affect these molecular patterns favorably and to enhance neoangiogenesis in old Wistar rats with hind-limb ischemia. At day 7 after surgery, HIF-1alpha and VEGF expression increased in the ischemic muscle of trained animals. Exercise increased capillary density and limb perfusion as revealed by histologic, angiographic, and dyed bead techniques. Furthermore, exercise capacity and limb trophism have significantly improved in trained aged rats. In these animals, the reduction of VEGF serum levels has reflected the comprehensive improvement in local ischemia evoked by exercise. In conclusion, prior exercise represents a valid tool to counteract age-related molecular alterations resulting in impaired angiogenesis in response to ischemia.
低氧诱导因子1（HIF-1）和血管内皮生长因子（VEGF）的下调被证明与年龄依赖性血管生成受损有关。在本研究中，我们探究预先进行运动是否能够有利地影响这些分子模式，并增强后肢缺血的老年Wistar大鼠的新生血管生成。术后第7天，训练动物缺血肌肉中的HIF-1α和VEGF表达增加。组织学、血管造影和染料微球技术显示，运动增加了毛细血管密度和肢体灌注。此外，训练的老年大鼠的运动能力和肢体营养状况有显著改善。在这些动物中，VEGF血清水平的降低反映了运动引起的局部缺血的全面改善。总之，预先进行运动是一种有效的手段，可对抗因年龄相关分子改变导致的缺血后血管生成受损。
