Martínez Paula, Agulló Loreine, Hernández Marcela, Seeger Michael
Laboratorio de Microbiología Molecular y Biotecnología Ambiental, Departamento de Química, Millennium Nucleus of Microbial Ecology, Universidad Técnica Federico Santa María, Av. España 1680, Valparaíso, Chile.
Arch Microbiol. 2007 Sep;188(3):289-97. doi: 10.1007/s00203-007-0247-4. Epub 2007 May 24.
Aerobic bacteria, such as Burkholderia xenovorans LB400, are able to degrade a wide range of polychlorobiphenyls (PCBs). Generally, these bacteria are not able to transform chlorobenzoates (CBAs), which accumulate during PCB degradation. In this study, the effects of CBAs on the growth, the morphology and the proteome of Burkholderia xenovorans LB400 were analysed. 4-CBA and 2-CBA were observed to inhibit the growth of strain LB400 on glucose. Strain LB400 exposed to 4-CBA exhibited increased number and size of electron-dense granules in the cytoplasm, which could be polyphosphates. Two-dimensional (2-D) polyacrylamide gel electrophoresis was used to characterise the molecular response of strain LB400 to 4-CBA. This compound induced the enzymes BenD and CatA of benzoate and catechol catabolic pathways. The induction of molecular chaperones DnaK and HtpG by 4-CBA indicated that the exposure to this compound constitutes a stressful condition for this bacterium. Additionally, the induction of some Krebs cycle enzymes was observed, probably as response to cellular energy requirements. This study contributes to the knowledge on the effects of CBA on the PCB-degrader Burkholderia xenovorans LB400.
需氧细菌，如嗜麦芽窄食单胞菌LB400，能够降解多种多氯联苯（PCBs）。一般来说，这些细菌无法转化在多氯联苯降解过程中积累的氯苯甲酸（CBAs）。在本研究中，分析了氯苯甲酸对嗜麦芽窄食单胞菌LB400的生长、形态和蛋白质组的影响。观察到4-氯苯甲酸和2-氯苯甲酸抑制了菌株LB400在葡萄糖上的生长。暴露于4-氯苯甲酸的菌株LB400在细胞质中电子致密颗粒的数量和大小增加，这些颗粒可能是多磷酸盐。二维（2-D）聚丙烯酰胺凝胶电泳用于表征菌株LB400对4-氯苯甲酸的分子反应。该化合物诱导了苯甲酸和儿茶酚分解代谢途径的酶BenD和CatA。4-氯苯甲酸对分子伴侣DnaK和HtpG的诱导表明，暴露于该化合物对这种细菌构成了应激条件。此外，还观察到一些三羧酸循环酶的诱导，这可能是对细胞能量需求的反应。本研究有助于了解氯苯甲酸对多氯联苯降解菌嗜麦芽窄食单胞菌LB400的影响。
