Agulló Loreine, Cámara Beatriz, Martínez Paula, Latorre Valeria, Seeger Michael
Laboratorio de Microbiología Molecular y Biotecnología Ambiental, Departamento de Química, Universidad Técnica Federico Santa María, Valparaíso, Chile.
FEMS Microbiol Lett. 2007 Feb;267(2):167-75. doi: 10.1111/j.1574-6968.2006.00554.x. Epub 2006 Dec 8.
We report the effects of 4-chlorobiphenyl and biphenyl on the physiology, morphology and proteome of the polychlorobiphenyl-degrader Burkholderia xenovorans LB400. The exposure to 4-chlorobiphenyl decreases the growth of LB400 on glucose, and cells exhibit irregular outer membranes, a larger periplasmic space and electron-dense granules in the cytoplasm. Additionally, lysis of cells was observed during incubation with 4-chlorobiphenyl or biphenyl. Proteome of B. xenovorans LB400 exposed to biphenyl and 4-chlorobiphenyl were analysed by two-dimensional gel electrophoresis. Besides induction of the Bph enzymes of biphenyl catabolic pathways, incubation with 4-chlorobiphenyl or biphenyl results in the induction of the molecular chaperones DnaK and GroEL. Induction of these chaperones, which were also induced during heat shock, strongly suggests that exposure to (chloro)biphenyls constitutes stress conditions for LB400. During growth of LB400 on biphenyl, oxidative stress was evidenced by the induction of alkyl hydroperoxide reductase AhpC, which was also induced during exposure to H(2)O(2). 4-chlorobiphenyl and biphenyl induced catechol 1,2-dioxygenase, as well as polypeptides involved in energy production, amino acid metabolism and transport.
我们报告了4-氯联苯和联苯对多氯联苯降解菌洋葱伯克霍尔德氏菌LB400的生理、形态和蛋白质组的影响。暴露于4-氯联苯会降低LB400在葡萄糖上的生长，细胞表现出不规则的外膜、更大的周质空间以及细胞质中的电子致密颗粒。此外，在与4-氯联苯或联苯孵育期间观察到细胞裂解。通过二维凝胶电泳分析了暴露于联苯和4-氯联苯的洋葱伯克霍尔德氏菌LB400的蛋白质组。除了诱导联苯分解代谢途径的Bph酶外，与4-氯联苯或联苯孵育还会导致分子伴侣DnaK和GroEL的诱导。这些伴侣蛋白在热休克期间也被诱导，这强烈表明暴露于（氯）联苯对LB400构成应激条件。在LB400在联苯上生长期间，烷基过氧化氢还原酶AhpC的诱导证明了氧化应激，该酶在暴露于H(2)O(2)期间也被诱导。4-氯联苯和联苯诱导了儿茶酚1,2-双加氧酶以及参与能量产生、氨基酸代谢和运输的多肽。
