Costas M, Morgan D D, Owen R J, Morgan D R
National Collection of Type Cultures, Central Public Health Laboratory, London.
Epidemiol Infect. 1991 Dec;107(3):607-17. doi: 10.1017/s095026880004930x.
Twenty-three pre- and post-treatment isolates of Helicobacter pylori from the antral mucosa of eight patients with dyspepsia and gastritis were compared using 1-D SDS PAGE of proteins. The protein patterns were highly reproducible and were used as the basis for two numerical analyses. The first, based on the total protein patterns, showed that a number of the strains did not cluster with their respective patient set. This was thought to be due to differences in both mobility and intensity of proteins in the major band region. The second analysis, based on partial patterns, excluding the major band region (51-68 kDa), divided the clinical isolates into clearly defined groups corresponding to the patient sets. Although there was a degree of heterogeneity with respect to protein pattern between the pre- and post-treatment isolates of some patients, there was nonetheless clear evidence that each patient was harbouring strains of only a single type. These results suggested that patients were not being reinfected with a different strain but that there was recrudescence of the pre-treatment strain. Protein 'fingerprints' provided a precise and reproducible means of strain differentiation, and revealed that in each patient the same strain persisted after drug therapy even though there was marked patient-to-patient strain variation.
运用蛋白质一维SDS聚丙烯酰胺凝胶电泳，对来自8例消化不良和胃炎患者胃窦黏膜的23株幽门螺杆菌治疗前后的分离株进行了比较。蛋白质图谱具有高度可重复性，并作为两种数值分析的基础。第一种分析基于总蛋白质图谱，结果显示许多菌株并未与其各自的患者组聚类。这被认为是由于主要条带区域蛋白质的迁移率和强度存在差异。第二种分析基于部分图谱，排除主要条带区域（51 - 68 kDa），将临床分离株分为与患者组相对应的明确界定的组。尽管一些患者治疗前后分离株的蛋白质图谱存在一定程度的异质性，但仍有明确证据表明每位患者仅携带单一类型的菌株。这些结果表明，患者并非被不同菌株再次感染，而是治疗前的菌株复发。蛋白质“指纹图谱”提供了一种精确且可重复的菌株区分方法，并揭示出尽管患者之间菌株存在显著差异，但药物治疗后每位患者体内持续存在的是同一菌株。
