Alexander M, Rahman M, Taylor M, Noble W C
Department of Skin Infection, United Medical School, Lambeth Hospital, London.
J Hosp Infect. 1988 Nov;12(4):273-87. doi: 10.1016/0195-6701(88)90069-2.
Forty-four isolates of Acinetobacter calcoaceticus var anitratus collected during hospital outbreaks were studied using polyacrylamide gel electrophoresis (PAGE), plasmid analysis, antibiograms and biochemical tests to determine their degree of similarity. Reproducibility tests were also carried out on the PAGE and biochemical techniques to determine their validity when used to compare bacteria of the same type isolated intermittently. PAGE data was analysed densitometrically and isolates compared using a similarity matrix. All methods were able to subdivide the isolates, but results did not always correlate well between methods. Reproducibility data indicated that careful attention to technique is required when organisms are examined by PAGE sequentially. Results suggest that no single biotyping technique is likely to be adequate and that electrophoretic, biochemical and antibiogram data may complement one another and other epidemiological data in the typing of these organisms.
对在医院感染暴发期间收集的44株醋酸钙不动杆菌硝酸盐阴性变种进行了研究，采用聚丙烯酰胺凝胶电泳（PAGE）、质粒分析、抗菌谱和生化试验来确定它们的相似程度。还对PAGE和生化技术进行了重复性测试，以确定在用于比较间歇性分离的同一类型细菌时它们的有效性。对PAGE数据进行了光密度分析，并使用相似性矩阵对分离株进行了比较。所有方法都能够对分离株进行细分，但不同方法的结果之间并不总是具有很好的相关性。重复性数据表明，当通过PAGE顺序检查生物体时，需要仔细注意技术。结果表明，没有一种单一的生物分型技术可能足够，电泳、生化和抗菌谱数据可能在这些生物体的分型中相互补充以及与其他流行病学数据相互补充。
