Owen R J, Costas M, Morgan D D, On S L, Hill L R, Pearson A D, Morgan D R
National Collection of Type Cultures, Central Public Health Laboratory, London, UK.
Antonie Van Leeuwenhoek. 1989 Mar;55(3):253-67. doi: 10.1007/BF00393854.
A total of 21 clinical isolates of Campylobacter pylori from Peru and the United Kingdom and two reference strains (from Australia), including the type strain (NCTC 11637T), were characterized by high resolution one-dimensional SDS-polyacrylamide gel electrophoresis of cellular proteins. The protein patterns contained more than 40 discrete bands and the approximate molecular weights of the major bands were 22, 27, 46, 57, 60, 65 and 93 kD. The total patterns were used as the basis of numerical analysis. Most strains were clustered in four phenons at 91% similarity with the exception of six ungrouped strains. Overall similarity was high with all strains linked in the phenogram at greater than or equal to 81%. Variation among strains was attributable principally to qualitative and quantitative band differences in the 47 to 56 kD (hypervariable) region of the C. pylori protein profile. From the analysis, ten different electropherotypes (EP-types) were identified. We demonstrated that differences were detectable among isolates from widely separated geographical locations as well as from the same location, although multiple isolates from two Peruvian patients had the same electropherotype. Our results indicate that determination of protein profiles provides the basis of a reproducible method for characterization of C. pylori isolates.
对来自秘鲁和英国的21株幽门螺杆菌临床分离株以及两株参考菌株（来自澳大利亚），包括模式菌株（NCTC 11637T），进行了细胞蛋白的高分辨率一维SDS聚丙烯酰胺凝胶电泳分析。蛋白图谱包含40多条离散条带，主要条带的近似分子量分别为22、27、46、57、60、65和93kD。总图谱用作数值分析的基础。除6株未分组菌株外，大多数菌株在相似度为91%时聚为4个表型群。在聚类图中，所有菌株的总体相似度较高，大于或等于81%。菌株间的差异主要归因于幽门螺杆菌蛋白图谱中47至56kD（高变）区域条带的定性和定量差异。通过分析，鉴定出10种不同的电泳型（EP型）。我们证明，尽管来自两名秘鲁患者的多个分离株具有相同的电泳型，但在来自广泛分离地理位置以及同一地点的分离株之间仍可检测到差异。我们的结果表明，蛋白图谱的测定为幽门螺杆菌分离株的鉴定提供了一种可重复方法的基础。
