Kim Yong-Deok, Kim Sung-Sik, Hwang Dae-Seok, Kim Sung-Gil, Kwon Yong-Hoon, Shin Sang-Hun, Kim Uk-Kyu, Kim Jong-Ryoul, Chung In-Kyo
Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Busan, Korea.
Lasers Surg Med. 2007 Jun;39(5):441-50. doi: 10.1002/lsm.20508.
This experiment using an animal experimental model was conducted in order to investigate the effect of low-level laser therapy (LLLT) on the healing of the dental titanium implant.
STUDY DESIGN/MATERIALS AND METHODS: The experimental group received LLLT for a week and the control group did not. Each group consisted of 10 rats. Two rats from the groups were euthenized on the days 1, 3, 7, 14, and 21 of the experiment. The expression of receptor activator of nuclear factor kB ligand (RANKL), osteoprotegerin (OPG), and receptor activator of nuclear factor kB (RANK) were investigated.
The expression of RANKL was observed from the initial stage of the installation of the implant for both the experimental and control groups. However, the degree of expression was higher in the experimental group. The degree of expression of OPG increased remarkably in the experimental group, while in the control group the degree of expression increased only slightly. In the experimental group, the expression of RANK was observed from the first day, but in the control group, it was weakly observed after day 3. The overall expression within the bone was slight on day 7 in the control group, while an active expression was observed in the experimental group. Bone density after installation of dental titanium implant during osseointegration in the experimental group was higher than the control group. The surface and structure of the titanium implant was not damaged by low-level laser (LLL).
From the above results, the expression of OPG, RANKL, and RANK during the osseointegration of the dental titanium implant was observed within bone tissue. The application of the LLL influenced the expression of OPG, RANKL, and RANK, and resulted in the expansion of metabolic bone activity and increased the activity of bone tissue cells.
本实验采用动物实验模型，旨在研究低强度激光疗法（LLLT）对牙科钛种植体愈合的影响。
研究设计/材料与方法：实验组接受为期一周的低强度激光疗法，对照组未接受。每组由10只大鼠组成。在实验的第1、3、7、14和21天，每组处死2只大鼠。研究核因子κB受体激活剂配体（RANKL）、骨保护素（OPG）和核因子κB受体激活剂（RANK）的表达。
实验组和对照组在种植体植入初期均观察到RANKL的表达。然而，实验组的表达程度更高。实验组OPG的表达程度显著增加，而对照组的表达程度仅略有增加。实验组在第一天就观察到RANK的表达，而对照组在第3天后才微弱观察到。对照组在第7天时骨内的总体表达轻微，而实验组则观察到活跃表达。实验组在骨整合过程中植入牙科钛种植体后的骨密度高于对照组。低强度激光（LLL）未对钛种植体的表面和结构造成损伤。
从上述结果可知，在骨组织内观察到牙科钛种植体骨整合过程中OPG、RANKL和RANK的表达。低强度激光的应用影响了OPG、RANKL和RANK的表达，导致代谢性骨活动扩大，增加了骨组织细胞的活性。
