Sasso Stuani Maria Bernadete, Sasso Stuani Andréa, Leite Pedroso Gabriela, da Silva Mira Paôla Caroline, Gollino Sara, Dastra Michel, Melsen Birte
Universidade de São Paulo, São Paulo, Brazil.
Aarhus University, Aarhus, Denmark.
Lasers Med Sci. 2025 May 27;40(1):245. doi: 10.1007/s10103-025-04497-6.
This study aimed to evaluate quantitatively the effects of low-level laser therapy (LLLT) on bone healing performed after rapid maxillary expansion (RME) in rats, using micro-computed tomography (micro-CT). Ninety rats were used, randomly distributed into three groups: control (n = 10) (young and adult rats - intact suture), experimental I (n = 40) (young and adult rats with RME without LLLT), and experimental II (n = 40) (young and adult rats with RME + LLLT). Five rats from the groups were euthanized on days 0, 7, 14, and 21 of the experiment and evaluated by microCT analysis. The laser increased the bone formation volume at 7 and 14 days (young rats) and 14 days (adult rats) were statistically significant (p < 0.05), but at the end of the healing (21 days), no statistically significant difference was observed between the laser and no-laser groups (p > 0.05). These findings suggest that LLLT improves bone mineralization during the early healing phases after RME, accelerating the process of bone mineralization during the initial experimental phase, which may have clinical implications in reducing orthodontic treatment duration and improving bone stability.
本研究旨在使用微型计算机断层扫描（micro-CT）定量评估低强度激光疗法（LLLT）对大鼠快速上颌扩弓（RME）后骨愈合的影响。共使用了90只大鼠，随机分为三组：对照组（n = 10）（年轻和成年大鼠 - 缝合完整）、实验组I（n = 40）（接受RME但未接受LLLT的年轻和成年大鼠）和实验组II（n = 40）（接受RME + LLLT的年轻和成年大鼠）。在实验的第0、7、14和21天，每组处死5只大鼠，并通过microCT分析进行评估。激光照射在第7天和14天（年轻大鼠）以及第14天（成年大鼠）增加了骨形成体积，差异具有统计学意义（p < 0.05），但在愈合末期（21天），激光组和非激光组之间未观察到统计学显著差异（p > 0.05）。这些发现表明，LLLT可改善RME后早期愈合阶段的骨矿化，在初始实验阶段加速骨矿化过程，这可能对缩短正畸治疗时间和提高骨稳定性具有临床意义。
