Piven' N N, Iliukhin V I, Zamarin A E, Alekseev V V, Vasil'ev V P
Zh Mikrobiol Epidemiol Immunobiol. 2007 Mar-Apr(2):49-53.
Sera of Burkholderia pseudomallei-infected golden hamsters, white mice, guinea pigs and white rats were studied. Immunochemical analysis revealed presence of antibodies against antigens 2, 3, 6, d, and g with significant predominance of antibodies to antigens 6 and d. Antigen d was detected irrespectively to strain used for experimental infection and experimental animal species. Antibodies to antigen 6 were detected only when strains belonging to Asian serovar, but not to Australian serovar, were used. On the basis of antigens 6 and d following methods of serologic diagnostics were developed: reaction of indirect hemagglutination, reaction of latex agglutination, and radioimmunological assay. Their sensitivity and specificity reached 100% during experimental melioidosis in golden hamsters, guinea pigs and white rats.
对感染类鼻疽伯克霍尔德菌的金黄仓鼠、小白鼠、豚鼠和大白鼠的血清进行了研究。免疫化学分析显示存在针对抗原2、3、6、d和g的抗体，其中针对抗原6和d的抗体占显著优势。无论用于实验感染的菌株和实验动物种类如何，均可检测到抗原d。仅当使用属于亚洲血清型而非澳大利亚血清型的菌株时，才可检测到针对抗原6的抗体。基于抗原6和d开发了以下血清学诊断方法：间接血凝反应、乳胶凝集反应和放射免疫测定。在金黄仓鼠、豚鼠和大白鼠的实验性类鼻疽病中，它们的敏感性和特异性均达到了100%。
