Phung L V, Han Y, Oka S, Hotta H, Smith M D, Theeparakun P, Yabuuchi E, Yano I
Department of Bacteriology, Osaka City University Medical School, Abeno-ku, Japan.
FEMS Immunol Med Microbiol. 1995 Dec;12(3-4):259-64. doi: 10.1111/j.1574-695X.1995.tb00201.x.
The serodiagnosis of melioidosis is commonly performed with tests using protein or polysaccharide as antigen. However, due to the low sensitivity, specificity and difficulty in the preparation of the antigens, more simple, precise and reproducible diagnostic tests were required. A purified glycolipid antigen (GL) which is a specific lipid component of Burkholderia pseudomallei has been used in an ELISA. With this antigen, specific immunoglobulin G (IgG) was detected in 49 out of 50 melioidosis sera. IgG was also detected in 2 out of 185 (Japanese) and 16 out of 181 (Vietnamese) control sera. Thus, the sensitivity was 98.0%, and specificity was 98.9% and 91.1% in the Japanese and Vietnamese sera, respectively. When the ELISA and indirect haemagglutination (IHA) tests were combined, a sensitivity of 100% and specificity of 97.8% were achieved. The advantages of the glycolipid antigen are ease of preparation, stability, high sensitivity and specificity.
类鼻疽的血清学诊断通常采用以蛋白质或多糖作为抗原的检测方法。然而，由于这些抗原的敏感性、特异性较低，且制备困难，因此需要更简单、精确且可重复的诊断检测方法。一种纯化的糖脂抗原（GL），它是类鼻疽杆菌的一种特异性脂质成分，已被用于酶联免疫吸附测定（ELISA）。使用这种抗原，在50份类鼻疽血清中有49份检测到特异性免疫球蛋白G（IgG）。在185份（日本）对照血清中有2份以及181份（越南）对照血清中有16份也检测到了IgG。因此，敏感性为98.0%，在日本和越南血清中的特异性分别为98.9%和91.1%。当将ELISA和间接血凝试验（IHA）结合使用时，敏感性达到100%，特异性为97.8%。糖脂抗原的优点包括易于制备、稳定性好、敏感性和特异性高。
