Chantratita Narisara, Wuthiekanun Vanaporn, Thanwisai Aunchalee, Limmathurotsakul Direk, Cheng Allen C, Chierakul Wirongrong, Day Nicholas P J, Peacock Sharon J
Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Clin Vaccine Immunol. 2007 Jan;14(1):110-3. doi: 10.1128/CVI.00289-06. Epub 2006 Nov 8.
Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).
为检测针对不同类鼻疽伯克霍尔德菌抗原制剂的抗体而开发的五种酶联免疫吸附测定法,作为泰国东北部类鼻疽病的诊断测试进行了评估。对于亲和纯化抗原(敏感性82%;特异性72%)和类鼻疽伯克霍尔德菌粗抗原(敏感性81%;特异性70%)观察到最高的诊断指数,相较于间接血凝试验(敏感性73%;特异性64%)有所改进。