Moseley Fleur L, Bicknell Katrina A, Marber Michael S, Brooks Gavin
School of Pharmacy, The University of Reading, Whiteknights, Reading, Berkshire, RG6 6AP, UK.
J Pharm Pharmacol. 2007 May;59(5):609-28. doi: 10.1211/jpp.59.5.0001.
The completion of the Human Genome Project has revealed a multitude of potential avenues for the identification of therapeutic targets. Extensive sequence information enables the identification of novel genes but does not facilitate a thorough understanding of how changes in gene expression control the molecular mechanisms underlying the development and regulation of a cell or the progression of disease. Proteomics encompasses the study of proteins expressed by a population of cells, and evaluates changes in protein expression, post-translational modifications, protein interactions, protein structure and splice variants, all of which are imperative for a complete understanding of protein function within the cell. From the outset, proteomics has been used to compare the protein profiles of cells in healthy and diseased states and as such can be used to identify proteins associated with disease development and progression. These candidate proteins might provide novel targets for new therapeutic agents or aid the development of assays for disease biomarkers. This review provides an overview of the current proteomic techniques available and focuses on their application in the search for novel therapeutic targets for the treatment of disease.
人类基因组计划的完成揭示了众多用于识别治疗靶点的潜在途径。丰富的序列信息能够识别新基因，但却无助于深入了解基因表达的变化如何控制细胞发育、调控以及疾病进展背后的分子机制。蛋白质组学涵盖了对一群细胞所表达蛋白质的研究，并评估蛋白质表达、翻译后修饰、蛋白质相互作用、蛋白质结构和剪接变体的变化，所有这些对于全面理解细胞内蛋白质功能而言都是必不可少的。从一开始，蛋白质组学就被用于比较健康状态和患病状态下细胞的蛋白质谱，因此可用于识别与疾病发展和进展相关的蛋白质。这些候选蛋白质可能为新型治疗药物提供新的靶点，或有助于开发疾病生物标志物检测方法。本综述概述了当前可用的蛋白质组学技术，并重点介绍了它们在寻找疾病治疗新靶点方面的应用。
