Use of insulin-like growth factor-1 to improve post-transfer survival of bovine embryos produced in vitro.

Use of in vitro produced embryos in commercial settings is limited by alterations in embryo function that result in reduced embryo and fetal survival and increased fetal, placental and neonatal abnormalities. One potential strategy to improve the efficiency of in vitro embryo production systems is to modify embryo culture conditions to more closely mimic embryo physiology in vivo. The milieu of the reproductive tract contains various growth factors and regulatory molecules which can regulate embryonic development. One of these molecules, insulin-like growth factor-1 (IGF-1), has been reported to have beneficial effects on bovine embryo development in vitro. Addition of IGF-1 to embryo culture can increase pregnancy and calving rates in lactating dairy cows. However, recent research indicates that such an effect is only observed when recipients are heat-stressed. In non-heat stressed animals, IGF-1 treatment does not affect conceptus length or interferon-tau secretion; thus, it is not likely that IGF-1 promotes embryo survival by blocking the luteolytic cascade. On the other hand, IGF-1 treatment can alter the relative abundance of certain developmentally important transcripts. Such differences may be important to improve embryo survival during heat stress. Further research into the effect of IGF-1 on gene expression and DNA methylation are warranted. Results indicate that modification of embryo culture with a growth factor can affect embryo survival after transfer. It is important that future research evaluating modifications to embryo culture also includes experiments involving transfer of embryos to recipients. Such experiments are important to demonstrate that proposed modifications actually improve post-transfer embryonic survival.