Velazquez M A, Newman M, Christie M F, Cripps P J, Crowe M A, Smith R F, Dobson H
Department of Veterinary Clinical Science, University of Liverpool, Leahurst, Neston, Cheshire, CH64 7TE, UK.
Theriogenology. 2005 Dec;64(9):1977-94. doi: 10.1016/j.theriogenology.2005.05.001. Epub 2005 Jun 4.
The objective was to determine if a single measurement of plasma insulin-like growth factor-1 (IGF-1) could predict the number of viable embryos obtained from donors and the likelihood of pregnancy in recipients in multiple ovulation and embryo transfer (MOET) programs in cattle. The embryo yields from 101 embryo recoveries were examined in maiden Holstein heifers (n=75) and multiparous Holstein cows (lactating cows n=20, dry cows n=6). Donors were super stimulated with FSH and embryo recovery was done non-surgically 7 days after artificial insemination. Embryos were classified according to the IETS criteria. Pregnancy rates in 100 maiden Holstein heifer recipients were analysed. Recipients were on day 7+/-1 of the estrous cycle at transfer. Pregnancy diagnosis was carried out at day 30 (PD 30) and rechecked at day 60 (PD 60) after transfer. Blood samples from coccygeal vessels taken at the time of embryo recovery (donors) and transfer (recipients) were analysed for IGF-1, insulin, beta-hydroxybutyrate (beta-OHB), non-esterified fatty acids (NEFA), urea and cholesterol. There was a negative correlation between the number of viable embryos and insulin (r=-0.33, P=0.025) in donor heifers. In donor cows, the number of viable embryos was correlated with IGF-1 (r=0.43, P=0.028) and cholesterol (r=-0.43, P=0.027). In recipients, PD30 and PD 60 were not affected by any of the circulating parameters analysed. Insulin, IGF-1 and cholesterol only explained 8.9, 13.9 and 15.8% of the variation in the production of viable embryos, respectively. Several factors affect MOET programs and under the circumstances of the present study the usefulness of hormonal and metabolic profiles as predictors of the outcome of this biotechnology was limited.
目的是确定单次测量血浆胰岛素样生长因子-1（IGF-1）是否能够预测在牛的多次排卵和胚胎移植（MOET）程序中，从供体获得的活胚数量以及受体怀孕的可能性。对101次胚胎回收所获胚胎产量进行了检查，涉及青年荷斯坦小母牛（n = 75）和经产荷斯坦奶牛（泌乳奶牛n = 20，干奶牛n = 6）。用促卵泡素（FSH）对供体进行超排刺激，并在人工授精7天后通过非手术方式回收胚胎。根据国际胚胎移植协会（IETS）标准对胚胎进行分类。分析了100头青年荷斯坦小母牛受体的妊娠率。受体在发情周期第7±1天进行胚胎移植。在移植后第30天（PD 30）进行妊娠诊断，并在第60天（PD 60）复查。对胚胎回收时（供体）和移植时（受体）从尾静脉采集的血样进行分析，检测IGF-1、胰岛素、β-羟基丁酸（β-OHB）、非酯化脂肪酸（NEFA）、尿素和胆固醇。在供体小母牛中，活胚数量与胰岛素呈负相关（r = -0.33，P = 0.025）。在供体奶牛中，活胚数量与IGF-1（r = 0.43，P = 0.028）和胆固醇（r = -0.43，P = 0.027）相关。在受体中，PD30和PD 60不受所分析的任何循环参数影响。胰岛素、IGF-1和胆固醇分别仅解释了活胚产量变异的8.9%、13.9%和15.8%。有几个因素会影响MOET程序，在本研究的情况下，激素和代谢谱作为这种生物技术结果预测指标的作用有限。
