乙二胺四乙酸对三氧化物矿物聚合体水化机制的影响。

Effects of EDTA on the hydration mechanism of mineral trioxide aggregate.

作者信息

Lee Y-L, Lin F-H, Wang W-H, Ritchie H H, Lan W-H, Lin C-P

机构信息

Graduate Institute of Clinical Dentistry, College of Medicine, National Taiwan University and National Taiwan University Hospital, No. 1 Chang-Te Street, Taipei 10016, Taiwan, ROC.

出版信息

J Dent Res. 2007 Jun;86(6):534-8. doi: 10.1177/154405910708600609.

DOI:10.1177/154405910708600609

PMID:17525353

Abstract

Ethylenediaminetetraacetic acid (EDTA) is commonly used during the preparation of obstructed root canals that face a high risk of root perforation. Such perforations may be repaired with mineral trioxide aggregate (MTA). Due to EDTA's ability to chelate calcium ions, we hypothesized that EDTA may disrupt the hydration of MTA. Using scanning electron microscopy and energy-dispersive x-ray spectroscopy, we found that MTA specimens stored in an EDTA solution had no crystalline structure and a Ca/Si molar ratio considerably lower than those obtained for specimens stored in distilled water and normal saline. Poor cell adhesion in EDTA-treated MTA was also noted. X-ray diffraction indicated that the peak corresponding to portlandite, which is normally present in hydrated MTA, was not shown in the EDTA group. The microhardness of EDTA-treated specimens was also significantly reduced (p < 0.0001). These findings suggest that EDTA interferes with the hydration of MTA, resulting in decreased hardness and poor biocompatibility.

摘要

乙二胺四乙酸（EDTA）常用于制备面临高根穿孔风险的阻塞根管。此类穿孔可用三氧化矿物凝聚体（MTA）修复。由于EDTA具有螯合钙离子的能力，我们推测EDTA可能会干扰MTA的水化。通过扫描电子显微镜和能量色散X射线光谱分析，我们发现储存在EDTA溶液中的MTA标本没有晶体结构，且钙/硅摩尔比远低于储存在蒸馏水和生理盐水中的标本。还注意到经EDTA处理的MTA细胞黏附性较差。X射线衍射表明，EDTA组未显示出通常存在于水化MTA中的对应于氢氧化钙的峰。经EDTA处理的标本的显微硬度也显著降低（p < 0.0001）。这些发现表明，EDTA会干扰MTA的水化，导致硬度降低和生物相容性差。