Department of Pediatric Dentistry, School and Hospital of Stomatology, China Medical University, 117 Nanjing North Street, Shenyang, 110002, China.
Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, China.
Clin Oral Investig. 2024 Jan 3;28(1):70. doi: 10.1007/s00784-023-05468-3.
To investigate in vitro effects of a nanoparticle bioceramic material, iRoot BP Plus, on stem cells from apical papilla (SCAP) and in vivo capacity to induce pulp-dentin complex formation.
The sealing ability of iRoot BP Plus was measured via scanning electron microscopy (SEM). SCAP were isolated and treated in vitro by iRoot BP Plus conditioned medium, with mineral trioxide aggregate (MTA) conditioned medium and regular medium used as controls, respectively. Cell proliferation was assessed by BrdU labeling and MTT assay and cell migration was evaluated with wound healing and transwell assays. Osteo/odontogenic potential was evaluated by Alizarin red S staining and qPCR. Pulp-dentin complex formation in vivo was assessed by a tooth slice subcutaneous implantation model.
iRoot BP Plus was more tightly bonded with the dentin. There was no difference in SCAP proliferation between iRoot BP Plus and control groups (P > 0.05). iRoot BP Plus had a greater capacity to elevated cell migration (P < 0.05) and osteo/odontogenic marker expression and mineralization nodule formation of SCAP compared with MTA groups (P < 0.05). Furthermore, the new continuous dentine layer and pulp-like tissue was observed in the iRoot BP Plus group in vivo.
iRoot BP Plus showed excellent sealing ability, promoted the migration and osteo/odontogenesis of SCAP and induced pulp-dentin complex formation without affecting the cell proliferation, which indicated iRoot BP Plus was a promising coronal sealing material in REPs.
The coronal sealing materials play crucial roles for the outcomes of REPs. This study showed that iRoot BP Plus has good coronal sealing and promote pulp-dentin complex formation compared with MTA, providing experimental evidences for the clinical application of iRoot BP Plus as a promising coronal seal material in REPs.
研究一种纳米生物陶瓷材料 iRoot BP Plus 对根尖乳头干细胞（SCAP）的体外作用及体内诱导牙髓-牙本质复合体形成的能力。
通过扫描电子显微镜（SEM）测量 iRoot BP Plus 的封闭能力。SCAP 被分离并在体外用 iRoot BP Plus 条件培养基处理，以矿物三氧化物聚合体（MTA）条件培养基和常规培养基分别作为对照。通过 BrdU 标记和 MTT 测定评估细胞增殖，通过划痕愈合和 Transwell 测定评估细胞迁移。通过茜素红 S 染色和 qPCR 评估成骨/成牙本质潜能。通过牙切片皮下植入模型评估体内牙髓-牙本质复合体的形成。
iRoot BP Plus 与牙本质的结合更紧密。iRoot BP Plus 组和对照组之间的 SCAP 增殖无差异（P > 0.05）。与 MTA 组相比，iRoot BP Plus 组具有更高的细胞迁移能力（P < 0.05），并能上调 SCAP 的成骨/成牙本质标志物表达和矿化结节形成（P < 0.05）。此外，在体内 iRoot BP Plus 组观察到新的连续牙本质层和牙髓样组织。
iRoot BP Plus 表现出优异的封闭能力，促进 SCAP 的迁移和成骨/成牙本质作用，并诱导牙髓-牙本质复合体形成，而不影响细胞增殖，这表明 iRoot BP Plus 是一种有前途的 REPs 冠方封闭材料。
冠方封闭材料对 REPs 的结果至关重要。本研究表明，与 MTA 相比，iRoot BP Plus 具有良好的冠方封闭和促进牙髓-牙本质复合体形成的能力，为 iRoot BP Plus 作为一种有前途的 REPs 冠方密封材料的临床应用提供了实验依据。
