Nagl Stefan, Wolfbeis Otto S
Institute of Analytical Chemistry, Chemo- and Biosensors University of Regensburg, 93040 Regensburg, Germany.
Analyst. 2007 Jun;132(6):507-11. doi: 10.1039/b702753b. Epub 2007 Apr 17.
Multiple optical sensors for chemical species are sensitive, non-toxic and non-invasive and enable spatially and temporally resolved multianalyte detection. Recent advances are highlighted with a focus on fluorescence-based methods and the biologically and clinically important analytes oxygen, pH, carbon dioxide and temperature. Indicator chemistries such as permeation-selective microbeads and nanoparticles allow the production of microscopically homogeneous sensor layers. The use of combinations of spectral discrimations along with time-resolved monitoring schemes based on luminescence lifetime or intensity-lifetime ratios enables all-optical real-time multianalyte determination.
用于化学物质的多种光学传感器具有灵敏、无毒且非侵入性的特点，能够实现空间和时间分辨的多分析物检测。重点介绍了基于荧光的方法以及生物学和临床上重要的分析物氧气、pH值、二氧化碳和温度方面的最新进展。诸如渗透选择性微珠和纳米颗粒等指示剂化学物质可用于制备微观均匀的传感器层。结合光谱鉴别以及基于发光寿命或强度-寿命比的时间分辨监测方案，能够实现全光学实时多分析物测定。
