Tehan Elizabeth C, Bukowski Rachel M, Chodavarapu Vamsy P, Titus Albert H, Cartwright Alexander N, Bright Frank V
Department of Chemistry, Natural Sciences Complex, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA.
Department of Electrical and Computer Engineering, McGill University, McConnell Engineering Building, 3480 University Street, Montreal, QC H3A 0E9, Canada.
Sensors (Basel). 2015 Jan 5;15(1):760-8. doi: 10.3390/s150100760.
We report a new strategy for generating a continuum of response profiles from a single luminescence-based sensor element by using phase-resolved detection. This strategy yields reliable responses that depend in a predictable manner on changes in the luminescent reporter lifetime in the presence of the target analyte, the excitation modulation frequency, and the detector (lock-in amplifier) phase angle. In the traditional steady-state mode, the sensor that we evaluate exhibits a linear, positive going response to changes in the target analyte concentration. Under phase-resolved conditions the analyte-dependent response profiles: (i) can become highly non-linear; (ii) yield negative going responses; (iii) can be biphasic; and (iv) can exhibit super sensitivity (e.g., sensitivities up to 300 fold greater in comparison to steady-state conditions).
我们报告了一种新策略，通过使用相分辨检测从单个基于发光的传感器元件生成连续的响应曲线。该策略产生可靠的响应，在存在目标分析物、激发调制频率和检测器（锁相放大器）相位角的情况下，这些响应以可预测的方式取决于发光报告分子寿命的变化。在传统的稳态模式下，我们评估的传感器对目标分析物浓度的变化表现出线性的正向响应。在相分辨条件下，与分析物相关的响应曲线：（i）可能变得高度非线性；（ii）产生负向响应；（iii）可能是双相的；（iv）可能表现出超灵敏度（例如，与稳态条件相比，灵敏度高达300倍）。
