Wang Cheng-long, Shi Da-zhuo, Yin Hui-jun
Department of Cardiology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2007 Apr;27(4):331-4.
To study the effect of Panax quinquefolius Saponin (PQS), an extraction from stem and leaf of American ginseng, on vascular regeneration in infarcted area, and expressions of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in myocardium of rats with acute myocardial infarction (AMI).
Fifty male Wistar rats were established into AMI model successfully and randomly divided into 5 groups equally, i.e. the model group, the high, middle and low dose PQS groups and the metoprolol group. They were treated with saline, different doses of PQS (54 mg/kg x d, 27 mg/kg x d, 13.5 mg/kg x d)and metoprolol (4.5 mg/kg x d) respectively, by gavage once a day for 14 days. Besides, a sham operated group and a normal control group were set up for control with 10 rats in each group. All rats were killed on the 15th day. Six samples of heart were chosen from each group for examining expressions of VEGF, bFGF and the VIII coagulation factor under light microscopy by immunohistochemical staining, and the quantitative analysis on positive responsive intensity of VEGF and bFGF was conducted on the other 4 heart samples using the image analysing system, then mean micro-vessel density (MMVD) was calculated.
The expressions of VEGF and MMVD were higher in the high and the middle dose PQS groups than those in the model group (P < 0.05) and the expression of bFGF was higher in the three PQS groups than that in the model group (P < 0.05).
PQS can protect myocardium from ischemic injury in rats after AMI by way of promoting angiogenesis in the infarcted or ischemic area of myocardium and up-regulating expressions of VEGF and bFGF in myocardial cells.
研究西洋参茎叶提取物西洋参皂苷（PQS）对急性心肌梗死（AMI）大鼠梗死区血管再生及心肌组织中血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）表达的影响。
将50只雄性Wistar大鼠成功制备成AMI模型，随机分为5组，每组10只，即模型组、PQS高、中、低剂量组及美托洛尔组。分别给予生理盐水、不同剂量PQS（54 mg/kg·d、27 mg/kg·d、13.5 mg/kg·d）和美托洛尔（4.5 mg/kg·d）灌胃，每日1次，连续14 d。此外，设假手术组和正常对照组，每组10只。于第15天处死所有大鼠。每组取6个心脏标本，采用免疫组织化学染色法在光学显微镜下检测VEGF、bFGF及Ⅷ因子表达，另取4个心脏标本用图像分析系统对VEGF和bFGF阳性反应强度进行定量分析，计算平均微血管密度（MMVD）。
PQS高、中剂量组VEGF表达及MMVD均高于模型组（P<0.05），3个PQS组bFGF表达均高于模型组（P<0.05）。
PQS可通过促进心肌梗死或缺血区血管生成及上调心肌细胞VEGF和bFGF表达，保护AMI大鼠心肌免受缺血损伤。
