Section of Sports Medicine, Beijing Sport University , Beijing, China.
Teaching Experiment Center, Beijing Sport University , Beijing, China.
J Sports Sci Med. 2014 Jan 20;13(1):15-21. eCollection 2014 Jan.
Bone-tendon junction (BTJ) injuries are common and may be caused by acute trauma and delayed healing during exercise or work. To understand the nature of the healing process of BTJ injuries would help to prevent injuries and improve treatment. Thirty-three mature female rabbit hindlimbs were assigned to normal control (CON, n = 7) and injury groups (n = 26). The acute injury was established by administering one 7 plum-blossom needle puncture. Specimens were harvested post injury at 1, 2, 4, and 8 weeks (ND1W, n = 6; ND2W, n = 6; ND4W, n = 7; and ND8W, n = 7). The injury existed in all of the injury groups. Compared with the CON group, all of the animals in the injury group showed poor cell profiles, an unclear or undetectable tide mark, a proteoglycan area and profile changes; the BTJ cell density diminished significantly in the ND1W (p < 0.01), ND2W (p < 0.05), ND4W (p < 0.01), and ND8W groups (p < 0.01); the fibrocartilage zone thickness in all injury groups was significantly thicker than in the CON group (p < 0.05), but no significant difference was found among the injury groups (p>0.05). The basic fibroblast growth factor (bFGF) expression in the CON group was significantly less than in the ND1W group (p<0.01), but no significant difference was found when compared with the ND2W, ND4W, and ND8W groups. The bFGF expression in the ND1W group was higher than that of the ND4W (p < 0.05) and ND8W groups (p < 0.01). The vascular endothelial growth factor (VEGF) levels were not significantly different among the groups (p > 0.05). The bFGF and VEGF expression levels indicated that the healing process stopped at 8 weeks post injury or was not activated, although the injury had not healed by histological examination. A repeatable animal model of BTJ acute injury was established in this study, and the results described the BTJ acute injury healing difficult concerned with the repairing stop. Key PointsThis study described the bone-tendon junction acute injury nature healing process.The bone-tendon junction acute injury could not be repaired naturally in 8 weeks.The bFGF and VEGF expression revealed that the bone-tendon junction acute injury delayed healing concern with the repairing stop.
骨-腱结合部（BTJ）损伤很常见，可能由急性创伤和运动或工作期间的延迟愈合引起。了解 BTJ 损伤的愈合过程的性质有助于预防损伤和改善治疗。将 33 只成熟雌性兔后腿分为正常对照组（CON，n = 7）和损伤组（n = 26）。急性损伤通过给予一次 7 个梅花针穿刺来建立。损伤后 1、2、4 和 8 周采集标本（ND1W，n = 6；ND2W，n = 6；ND4W，n = 7；和 ND8W，n = 7）。所有损伤组均存在损伤。与 CON 组相比，损伤组的所有动物均表现出较差的细胞形态，潮痕不清或无法检测，糖胺聚糖区和形态发生变化；BTJ 细胞密度在 ND1W（p < 0.01）、ND2W（p < 0.05）、ND4W（p < 0.01）和 ND8W 组中显著降低（p < 0.01）；所有损伤组的纤维软骨带厚度均明显大于 CON 组（p < 0.05），但各组之间无显著差异（p>0.05）。CON 组的碱性成纤维细胞生长因子（bFGF）表达明显低于 ND1W 组（p<0.01），但与 ND2W、ND4W 和 ND8W 组无显著差异。ND1W 组的 bFGF 表达高于 ND4W（p < 0.05）和 ND8W 组（p < 0.01）。各组血管内皮生长因子（VEGF）水平无显著差异（p > 0.05）。bFGF 和 VEGF 的表达水平表明，尽管组织学检查显示损伤尚未愈合，但损伤后 8 周的愈合过程停止或未被激活。本研究建立了一种可重复的 BTJ 急性损伤动物模型，结果描述了 BTJ 急性损伤愈合困难与修复停止有关。
本研究描述了 BTJ 急性损伤的自然愈合过程。
8 周内 BTJ 急性损伤不能自然修复。
bFGF 和 VEGF 的表达表明 BTJ 急性损伤延迟愈合与修复停止有关。
