Fouz Belén, Roig Francisco J, Amaro Carmen
Department of Microbiology and Ecology, Faculty of Biology, University of Valencia, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain.
Microbiology (Reading). 2007 Jun;153(Pt 6):1926-1934. doi: 10.1099/mic.0.2006/005405-0.
Vibrio vulnificus is a bacterial species that is virulent for humans and fish. Human isolates are classified into biotypes 1 and 3 (BT1 and BT3) and fish isolates into biotype 2 (BT2). However, a few human infections caused by BT2 isolates have been reported worldwide (zoonosis). These BT2 human isolates belong to serovar E (SerE), which is also present in diseased fish. The aim of the present work was to characterize a new BT2 serovar [serovar A (SerA)], which emerged in the European fish-farming industry in 2000, by means of phenotypic, serological and genetic [plasmid profiling, ribotyping and random amplified polymorphic DNA (RAPD)] methodologies. The results confirmed that SerA constitutes a homogeneous O-serogroup within the species that shares plasmidic information with SerE. Like SerE, this new serogroup was resistant to fresh fish serum, as well as being highly virulent for fish. In contrast, it was sensitive to human serum and avirulent for mice, even after pretreatment with iron. The two serovars presented different biochemical profiles as well as specific patterns by ribotyping and RAPD analysis. In conclusion, SerA seems to constitute a different clonal group that has recently emerged within the species V. vulnificus, with pathogenic potential for fish but not for humans.
创伤弧菌是一种对人类和鱼类具有致病性的细菌。从人类身上分离出的菌株分为生物型1和生物型3（BT1和BT3），从鱼类身上分离出的菌株分为生物型2（BT2）。然而，全球范围内已有一些由BT2菌株引起的人类感染病例报道（人畜共患病）。这些BT2人类分离株属于血清型E（SerE），患病鱼类中也存在该血清型。本研究的目的是通过表型、血清学和遗传学方法[质粒图谱分析、核糖体分型和随机扩增多态性DNA（RAPD）]对2000年出现在欧洲养鱼业中的一种新的BT2血清型[血清型A（SerA）]进行特征描述。结果证实，SerA在该物种中构成一个同质的O血清群，与SerE共享质粒信息。与SerE一样，这个新的血清群对新鲜鱼血清具有抗性，并且对鱼类具有高度致病性。相比之下，它对人血清敏感，对小鼠无致病性，即使经过铁预处理也是如此。这两种血清型呈现出不同的生化特征以及核糖体分型和RAPD分析的特定模式。总之，SerA似乎构成了一个不同的克隆群，它最近在创伤弧菌物种中出现，对鱼类具有致病潜力，但对人类没有致病潜力。
