Departamento de Microbiología, Facultad de Biología, Universidad de Valencia, Valencia 46100, Spain.
Appl Environ Microbiol. 2010 Mar;76(5):1328-33. doi: 10.1128/AEM.01042-09. Epub 2009 Dec 28.
Vibrio vulnificus is a heterogeneous species that comprises strains virulent and avirulent for humans and fish, and it is grouped into three biotypes. In this report, we describe a PCR-based methodology that allows both the species identification and discrimination of those isolates that could be considered dangerous to public health. Discrimination is based on the amplification of a variable region located within the gene pilF, which seems to be associated with potential human pathogenicity, regardless of the biotype of the strain.
创伤弧菌是一个具有多样性的物种,其中包含对人类和鱼类具有毒力和无毒性的菌株,并且它被分为三个生物型。在本报告中,我们描述了一种基于 PCR 的方法,该方法既可以鉴定物种,又可以区分那些被认为对公共健康有危险的分离株。这种区分是基于扩增位于 pilF 基因内的一个可变区域,该区域似乎与潜在的人类致病性相关,而与菌株的生物型无关。