Lucier G W, Sonawane B R, McDaniel O S
Drug Metab Dispos. 1977 May-Jun;5(3):279-87.
The relative activities of uridine diphosphoglucuronyltransferase (UDPGT) and beta-glucuronidase (betaG) were measured during perinatal development of hepatic and extrahepatic tissues to determine the balance between glucuronidation and deglucuronidation reactions at different developmental stages. Liver, lung, kidney, intestine, and placenta were studied in guinea pigs and rabbits. In general, betaG activities exceeded those of UDPGT in fetal tissues, whereas the converse was evident in adults. There were significant species and age differences in the onset of betaG and UDPGT activities and the occurrence of developmental peaks. A dramatic betaG developmental peak was observed in fetal guinea pig intestine and newborn rabbit intestine. Both microsomal and lysosomal betaG exhibited similar developmental patterns in all tissues tested. Hepatic nonsteroid UDPGT activities were higher at parturition than in adult animals, whereas no such developmental peak occurred for steroid UDPGT. Triton X-100 activated fetal UDPGT in vitro by approximately the same factor as it did for adult UDPGT.
在肝脏和肝外组织的围产期发育过程中，测定了尿苷二磷酸葡萄糖醛酸基转移酶（UDPGT）和β-葡萄糖醛酸酶（βG）的相对活性，以确定不同发育阶段葡萄糖醛酸化和去葡萄糖醛酸化反应之间的平衡。在豚鼠和兔子中研究了肝脏、肺、肾脏、肠道和胎盘。一般来说，胎儿组织中βG的活性超过UDPGT，而在成年动物中情况则相反。βG和UDPGT活性的起始以及发育高峰的出现存在显著的物种和年龄差异。在胎儿豚鼠肠道和新生兔子肠道中观察到显著的βG发育高峰。在所有测试组织中，微粒体和溶酶体βG均呈现相似的发育模式。肝脏非甾体UDPGT活性在分娩时高于成年动物，而甾体UDPGT则未出现这种发育高峰。Triton X-100在体外激活胎儿UDPGT的程度与激活成年UDPGT的程度大致相同。
