Department of Environmental Health Sciences, University of Michigan, Ann Arbor, MI 48109, USA.
J Biochem Mol Toxicol. 2013 Feb;27(2):116-23. doi: 10.1002/jbt.21459. Epub 2012 Dec 3.
Widespread exposure to the endocrine active compound, bisphenol A (BPA), is well documented in humans. A growing body of literature suggests adverse health outcomes associated with varying ranges of exposure to BPA. In the current study, we measured the internal dose of free BPA and conjugated BPA and evaluated gene expression of biotransformation enzymes specific for BPA metabolism in 50 first- and second-trimester human fetal liver samples. Both free BPA and conjugated BPA concentrations varied widely, with free BPA exhibiting three times higher concentrations than conjugated BPA concentrations. As compared to gender-matched adult liver controls, UDP-glucuronyltransferase, sulfotransferase, and steroid sulfatase genes exhibited reduced expression whereas β-glucuronidase mRNA expression remained unchanged in the fetal tissues. This study provides evidence that there is considerable exposure to BPA during human pregnancy and that the capacity for BPA metabolism is altered in the human fetal liver.
广泛接触内分泌活性化合物双酚 A（BPA）在人类中已有充分记录。越来越多的文献表明，接触 BPA 的范围不同会对健康产生不良影响。在本研究中，我们测量了 50 个人类胎儿肝脏样本中游离 BPA 和结合 BPA 的内剂量，并评估了针对 BPA 代谢的生物转化酶的基因表达。游离 BPA 和结合 BPA 的浓度差异很大，游离 BPA 的浓度是结合 BPA 浓度的三倍。与性别匹配的成人肝脏对照相比，UDP-葡萄糖醛酸基转移酶、磺基转移酶和甾体硫酸酯酶基因的表达降低，而胎儿组织中β-葡萄糖醛酸酶 mRNA 的表达保持不变。本研究提供的证据表明，人类妊娠期间存在大量的 BPA 暴露，而人类胎儿肝脏中 BPA 代谢的能力发生了改变。
