Takashi Hoshiba, Katsumi Mochitate, Toshihiro Akaike
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
Biochem Biophys Res Commun. 2007 Jul 20;359(1):151-6. doi: 10.1016/j.bbrc.2007.05.079. Epub 2007 May 22.
To establish liver tissue engineering, the effective substratum for hepatocytes culture should be developed. Up to now, it is believed that Matrigel, which contains several basement membrane proteins produced by sarcoma cells, is the most effective substratum. Matrigel does not contain extracellular matrix molecules derived from epithelial cells although the space of Disse contains the molecules such as laminin-511/521 (laminin-10/11). Therefore, the basement membrane formed by epithelial cells can be more effective substratum than Matrigel. In this study, we evaluated hepatocytes behavior on basement membrane (rBM) formed by alveolar epithelial cells. The viability of hepatocytes on rBM is higher than that of Matrigel within 5 days. Also, the expression of Cyp1a2 induced by beta-naphthoflavone can be observed in hepatocytes on rBM but not in Matrigel. These results indicate that rBM is a more effective substratum for hepatocyte culture than Matrigel.
为建立肝脏组织工程，应开发用于肝细胞培养的有效基质。到目前为止，人们认为基质胶是最有效的基质，它含有肉瘤细胞产生的几种基底膜蛋白。尽管狄氏间隙含有层粘连蛋白-511/521（层粘连蛋白-10/11）等分子，但基质胶并不包含源自上皮细胞的细胞外基质分子。因此，由上皮细胞形成的基底膜可能是比基质胶更有效的基质。在本研究中，我们评估了肝细胞在肺泡上皮细胞形成的基底膜（rBM）上的行为。在5天内，肝细胞在rBM上的活力高于在基质胶上的活力。此外，在rBM上的肝细胞中可观察到β-萘黄酮诱导的Cyp1a2表达，而在基质胶上的肝细胞中则未观察到。这些结果表明，rBM是比基质胶更有效的肝细胞培养基质。
