Department of Human Anatomy and Embryology, Faculty of Medicine, University of Granada, Granada, 18016, Spain.
Instituto de Investigación Biosanitaria ibs. GRANADA, University of Granada, Granada, 18014, Spain.
Adv Healthc Mater. 2021 Apr;10(8):e2001847. doi: 10.1002/adhm.202001847. Epub 2021 Mar 1.
The use of decellularized extracellular matrix (dECM) as a biomaterial has been an important step forward for the development of functional tissue constructs. In addition to tissues and organs, cell cultures are gaining a lot of attention as an alternative source of dECM. In this work, a novel biomimetic hydrogel is developed based on dECM obtained from mesenchymal stem cells (mdECM) for cartilage tissue engineering. To this end, cells are seeded under specific culture conditions to generate an early chondrogenic extracellular matrix (ECM) providing cues and elements necessary for cartilage development. The composition is determined by quantitative, histological, and mass spectrometry techniques. Moreover, the decellularization process is evaluated by measuring the DNA content and compositional analyses, and the hydrogel is formulated at different concentrations (3% and 6% w/v). Results show that mdECM derived hydrogels possess excellent biocompatibility and suitable physicochemical and mechanical properties for their injectability. Furthermore, it is evidenced that this hydrogel is able to induce chondrogenesis of mesenchymal stem cells (MSCs) without supplemental factors and, furthermore, to form hyaline cartilage-like tissue after in vivo implantation. These findings demonstrate for the first time the potential of this hydrogel based on mdECM for applications in cartilage repair and regeneration.
使用去细胞细胞外基质 (dECM) 作为生物材料是开发功能性组织构建体的重要一步。除了组织和器官，细胞培养作为 dECM 的替代来源也受到了广泛关注。在这项工作中，基于间充质干细胞 (mdECM) 开发了一种新型仿生水凝胶，用于软骨组织工程。为此，在特定的培养条件下接种细胞，以生成早期软骨细胞外基质 (ECM)，为软骨发育提供必要的线索和元素。通过定量、组织学和质谱技术确定组成。此外，通过测量 DNA 含量和组成分析来评估脱细胞过程，并在不同浓度（3%和 6%w/v）下配制水凝胶。结果表明，mdECM 衍生的水凝胶具有出色的生物相容性和适合注射的理化和机械性能。此外，有证据表明，这种水凝胶能够在没有补充因子的情况下诱导间充质干细胞 (MSCs) 的软骨生成，并在体内植入后形成透明软骨样组织。这些发现首次证明了基于 mdECM 的这种水凝胶在软骨修复和再生中的应用潜力。
