Kanda Yasukazu, Yokotani Naoki, Maeda Satoru, Nishizawa Yoko, Kamakura Takashi, Mori Masaki
a Institute of Agrobiological Sciences, NARO (NIAS) , Tsukuba , Japan.
b Graduate School of Science and Technology , Tokyo University of Science , Noda , Japan.
Biosci Biotechnol Biochem. 2017 Aug;81(8):1497-1502. doi: 10.1080/09168451.2017.1325710. Epub 2017 May 18.
Broad-Spectrum Resistance 1 (BSR1) encodes a rice receptor-like cytoplasmic kinase, and enhances disease resistance when overexpressed. Rice plants overexpressing BSR1 are highly resistant to diverse pathogens, including rice blast fungus. However, the mechanism responsible for this resistance has not been fully characterized. To analyze the BSR1 function, BSR1-knockout (BSR1-KO) plants were generated using a clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system. Experiments using suspension-cultured cells revealed that defense responses including HO production (i.e. oxidative burst) and expression of defense-related genes induced by autoclaved conidia of the rice blast fungus significantly decreased in BSR1-KO cells. Furthermore, a treatment with chitin oligomers which function as microbe-associated molecular patterns (MAMPs) of the rice blast fungus resulted in considerably suppressed defense responses in BSR1-KO cells. These results suggest that BSR1 is important for the rice innate immunity triggered by the perception of chitin.
广谱抗性1（BSR1）编码一种水稻类受体胞质激酶，过表达时可增强抗病性。过表达BSR1的水稻植株对多种病原体具有高度抗性，包括稻瘟病菌。然而，这种抗性的机制尚未完全阐明。为了分析BSR1的功能，利用成簇规律间隔短回文重复序列（CRISPR）/CRISPR相关蛋白9（Cas9）系统构建了BSR1基因敲除（BSR1-KO）植株。利用悬浮培养细胞进行的实验表明，在BSR1-KO细胞中，包括过氧化氢产生（即氧化爆发）和由稻瘟病菌高压灭菌分生孢子诱导的防御相关基因表达在内的防御反应显著降低。此外，用作为稻瘟病菌微生物相关分子模式（MAMPs）的几丁质寡聚物处理导致BSR1-KO细胞中的防御反应受到显著抑制。这些结果表明，BSR1对于由几丁质感知触发的水稻先天免疫很重要。
