Drury G I, Yukna R A
Periodontics Department, Louisiana State University School of Dentistry, New Orleans, LA.
J Periodontol. 1991 Nov;62(11):652-8. doi: 10.1902/jop.1991.62.11.652.
Tetracyclines (TTC) have been used both systemically and locally during periodontal bone grafting procedures, but previous work regarding the effect of TTC on new bone formation has been contradictory. The purpose of this study was to determine if the use of locally applied TTC in combination with freeze-dried bone allografts (FDBA) would enhance bone regeneration in an experimental alveolar bone osseous defect grafting system. Pre-weighed freeze-dried bone allograft particles (300-500 mu diameter) were placed in nylon mesh chambers (250 mu pore size) and rehydrated with either 10 micrograms/ml aqueous TTC or sterile distilled water. Empty chambers were used as further controls. The chambers were placed in the posterior mandible of baboons in surgically created windows. After 3 and 5 weeks, the chambers were retrieved, processed histologically, and analyzed histometrically for new bone formation. The TTC-rehydrated FDBA demonstrated much greater (greater than 5x) new bone formation than the water rehydrated FDBA. These results strongly suggest that locally-applied TTC in combination with FDBA enhances new bone formation in experimental alveolar bone defects.
四环素（TTC）已在牙周骨移植手术中全身和局部使用，但先前关于TTC对新骨形成影响的研究结果相互矛盾。本研究的目的是确定在实验性牙槽骨骨缺损移植系统中，局部应用TTC联合冻干异体骨（FDBA）是否会增强骨再生。将预先称重的冻干异体骨颗粒（直径300 - 500微米）置于尼龙网室（孔径250微米）中，并用10微克/毫升的TTC水溶液或无菌蒸馏水复水。空室用作进一步对照。将这些室放置在狒狒下颌后部手术创建的窗口中。3周和5周后，取出这些室，进行组织学处理，并进行组织计量学分析以评估新骨形成情况。用TTC复水的FDBA显示出比用水复水的FDBA多得多（大于5倍）的新骨形成。这些结果强烈表明，局部应用TTC联合FDBA可增强实验性牙槽骨缺损中的新骨形成。
