Pinotti Matheus Fécchio, Silva Maeli Dal-Pai, Sugizaki Mário Mateus, Novelli Yeda Santana Diniz, Sant'ana Lea Sílvia, Aragon Flávio Ferrari, Padovani Carlos Roberto, Novelli Ethel Lourenzi Barbosa, Cicogna Antonio Carlos
Faculdade Médica, Faculdade de Medicina de Botucatu, UNESP, Botucatu, SP, Brazil.
Arq Bras Cardiol. 2007 Mar;88(3):346-53. doi: 10.1590/s0066-782x2007000300015.
To study the influence of saturated (SFA) and unsaturated fatty acid (UFA) rich diets on mechanical function, morphology and oxidative stress in rat myocardium.
Male, 60-day-old Wistar rats were fed a control (n=8), a SFA (n=8), or a UFA-rich diet (n=8) for sixty days. Mechanical function was studied in isolated left ventricle papillary muscle under isometric and isotonic contractions, in basal conditions (1.25 mM calcium chloride) and after 5.2 mM calcium chloride and beta-adrenergic stimuli with 1.0 microM isoproterenol. Left ventricle fragments were used to study oxidative stress and morphology under light and electron microscopy.
SFA and UFA-rich diets did not change myocardium mechanical function. Both diets caused oxidative stress, with high lipid hydroperoxide and low superoxide-dismutase concentrations. UFA rich diet decreased catalase expression and SFA rich diet decreased the amount of myocardial glutathione-peroxidase. Both diets promoted light ultrastructural injuries such as lipid deposits and cell membrane injuries.
Results suggest that SFA and UFA rich diets do not alter isolated muscle mechanical function, but promote light myocardial morphological injuries and oxidative stress.
研究富含饱和脂肪酸（SFA）和不饱和脂肪酸（UFA）的饮食对大鼠心肌机械功能、形态和氧化应激的影响。
将60日龄雄性Wistar大鼠分为对照组（n = 8）、SFA组（n = 8）或富含UFA组（n = 8），喂养60天。在等长和等张收缩条件下，于基础状态（1.25 mM氯化钙）以及5.2 mM氯化钙和1.0 microM异丙肾上腺素β-肾上腺素能刺激后，对分离的左心室乳头肌的机械功能进行研究。使用左心室片段在光学和电子显微镜下研究氧化应激和形态。
富含SFA和UFA的饮食并未改变心肌的机械功能。两种饮食均导致氧化应激，脂质过氧化氢浓度高，超氧化物歧化酶浓度低。富含UFA的饮食降低了过氧化氢酶的表达，富含SFA的饮食降低了心肌谷胱甘肽过氧化物酶的含量。两种饮食均促进了轻度超微结构损伤，如脂质沉积和细胞膜损伤。
结果表明，富含SFA和UFA的饮食不会改变离体肌肉的机械功能，但会促进轻度心肌形态损伤和氧化应激。
